Placental release of distinct DNA-associated micro-particles into maternal circulation: reflective of gestation time and preeclampsia.
Placental release of distinct DNA-associated micro-particles into maternal circulation: reflective of gestation time and preeclampsia.
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DOI：
10.1016/j.placenta.2009.06.012
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发表时间：
2009-10
期刊：
影响因子：
3.8
通讯作者：
Lewis, D. E.
中科院分区：
文献类型：
--
作者：
Orozco, A. F.;Jorgez, C. J.;Ramos-Perez, W. D.;Popek, E. J.;Yu, X.;Kozinetz, C. A.;Bischoff, F. Z.;Lewis, D. E.
关键词：
本研究的目的是确定母体血浆中dna相关微粒（MPs）是否表达胎源性人白细胞抗原g （HLA-G）或胎盘碱性磷酸酶（PLAP），以及正常妊娠和子痫前期妇女的水平是否不同。用流式细胞术检测正常孕妇和子痫前期患者血浆中表达HLA-G或PLAP的dna相关MPs。与未怀孕对照组相比，母体血浆中dna相关的HLA-G+ MPs显著增加（p < 0.005），在妊娠早期和中期水平最高。与未怀孕对照组相比，母体血浆中dna相关PLAP+ MPs也增加（p < 0.006），在妊娠中期和晚期水平最高。足月子痫前期妇女的dna相关MPs水平高于对照组孕妇。子痫前期妇女血浆HLA-G+ MPs比正常孕妇血浆HLA-G+ MPs每MP含有更多的DNA （p < 0.03）。HLA-G和PLAP MPs在妊娠不同时期母体循环增加。每HLA-G+ MP的DNA量在子痫前期妇女中增加，这可能表明细胞外滋养细胞功能失调。
The aim of this study was to determine whether DNA-associated micro-particles (MPs) in maternal plasma express fetal-derived human leukocyte antigen-G (HLA-G) or placental alkaline phosphatase (PLAP) and whether the levels differ between women with normotensive pregnancies and preeclampsia. DNA-associated MPs expressing HLA-G or PLAP were examined in the plasma of normal pregnant women and preeclamptic patients using flow cytometric analysis. DNA-associated HLA-G+ MPs were significantly increased in maternal plasma compared to plasma from non-pregnant controls (p < 0.005), with highest levels found in first and second trimesters. DNA-associated PLAP+ MPs were also increased in maternal plasma compared to plasma from non-pregnant controls (p < 0.006), with highest levels in second and third trimesters. Term preeclamptic women had higher levels of DNA-associated MPs than control pregnant women. HLA-G+ MPs from the plasma of preeclamptic women had more DNA per MP than HLA-G+ MPs from the plasma of normal pregnant women (p < 0.03). HLA-G and PLAP MPs increase in maternal circulation at different times during gestation. DNA amounts per HLA-G+ MP increase in preeclamptic women which might indicate dysfunctional extravillous cytotrophoblasts.
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