PROTEOLYSIS OF TAU BY CALPAIN
PROTEOLYSIS OF TAU BY CALPAIN
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DOI：
10.1016/0006-291x(89)91150-9
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发表时间：
1989-09-29
影响因子：
3.1
通讯作者：
BINDER, LI
中科院分区：
文献类型：
--
作者：
JOHNSON, GVW;JOPE, RS;BINDER, LI
研究了与两次循环微管或总脑热稳定部分相关的 tau 蛋白诱导的 tau 蛋白水解。两次循环的微管tau蛋白被钙蛋白酶快速水解。相比之下，从总脑热稳定性部分纯化的 tau 蛋白对钙蛋白酶的降解具有很强的抵抗力。这些结果清楚地表明，大脑中至少存在两种基于钙蛋白酶敏感性的 tau 蛋白群体，一种是与微管相关的钙蛋白酶敏感形式，另一种是钙蛋白酶抗性形式，可能代表大脑中的另一种 tau 蛋白群体。
The calpain)-induced proteolysis of tau associated with twice-cycled microtubules or from a total brain heat-stable fraction was studied. Twice-cycled microtubule tau was rapidly hydrolyzed by calpain. In contrast, tau purified from the total brain heat-stable fraction was very resistant to degradation by calpain. These results clearly demonstrate that there are at least 2 populations of tau in the brain based on calpain-sensitivity, a calpain-sensitive form that is associated with microtubules and a calpain-resistant form that may represent another population of tau in the brain.