Synergism between porcine reproductive and respiratory syndrome virus (PRRSV) and Salmonella choleraesuis in swine
Synergism between porcine reproductive and respiratory syndrome virus (PRRSV) and Salmonella choleraesuis in swine
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DOI：
10.1016/s0378-1135(99)00175-3
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发表时间：
2000-02-01
影响因子：
3.3
通讯作者：
Zimmerman, JJ
中科院分区：
文献类型：
--
作者：
Wills, RW;Gray, JT;Zimmerman, JJ
猪繁殖与呼吸综合征病毒(PRRSV)和猪霍乱沙门氏菌是养猪业造成经济损失的两大主要原因。虽然呼吸道疾病在霍乱沙门氏菌和PRRSV感染中都很常见，但导致其发展的因素在很大程度上仍不确定。我们研究了猪繁殖与呼吸综合征病毒、霍乱沙门氏菌和应激反应在5周龄猪中的相互作用。在两个独立试验中，使用三个因素的所有组合(第0天接种霍乱沙门氏菌，第3天接种PRRSV，第3-7天地塞米松治疗)，产生8个处理组。采集粪便、扁桃体和鼻拭子、血清和死后组织进行细菌学和病毒学检查。仅接种猪繁殖与呼吸综合征病毒或仅接种S霍乱弧菌的猪未观察到临床症状。相比之下，S、霍乱和PRRSV双重感染的猪表现出节俭、毛发粗糙、呼吸困难和腹泻，这三种处理因素的猪受到的影响最严重，这组动物中43%(3/7)死亡。与其他处理组相比，这一组中的个体在粪便中排出的霍乱沙门氏菌数量显著增加，血清PRRSV滴度显著增加(p<或等于0.05)。此外，霍乱沙门氏菌和PRRSV在接种后第21天从该组更多的组织中恢复，且该组的霍乱弧菌和PRRSV排出的时间和猪的数量比其他组多。这些结果表明，PRRSV、霍乱沙门氏菌和地塞米松协同作用，产生了与现场观察到的症状相似的症状。(C)2000 Elsevier Science B.V.保留所有权利。
Porcine reproductive and respiratory syndrome virus (PRRSV) and Salmonella choleraesuis are two leading causes of economic loss in the swine industry. While respiratory disease is common in both S. choleraesuis and PRRSV infections, the factors that contribute to its development remain largely undefined. We investigated the interaction of PRRSV, S. choleraesuis, and stress in 5-week-old swine. All combinations of three factors (inoculation with S. choleraesuis on Day 0, PRRSV on Day 3, and treatment with dexamethasone on Days 3-7) were used to produce eight treatment groups in two independent trials. Fecal samples, tonsil and nasal swabs, serum samples and postmortem tissues were collected for bacteriologic and virologic examinations. No clinical signs were observed in pigs inoculated with only PRRSV or only S, choleraesuis. In contrast, pigs which were dually infected with S, choleraesuis and PRRSV exhibited unthriftiness, rough hair coats, dyspnea, and diarrhea, The pigs which received all three treatment factors were the most severely affected and 43% (three of seven) of the animals in this group died. Individuals in this group shed significantly higher quantities of S. choleraesuis in feces and had significantly higher serum PRRSV titers compared to other treatments (p less than or equal to 0.05). In addition, S. choleraesuis and PRRSV were shed longer and by more pigs in this group than other groups and S. choleraesuis was recovered from more tissues in this group on Day 21 post inoculation. These results suggested that PRRSV, S. choleraesuis, and dexamethasone acted synergistically to produce a syndrome similar to that observed in the field. (C) 2000 Elsevier Science B.V. All rights reserved.