Knockdown of NFBD1/MDC1 enhances chemosensitivity to cisplatin or 5-fluorouracil in nasopharyngeal carcinoma CNE1 cells
Knockdown of NFBD1/MDC1 enhances chemosensitivity to cisplatin or 5-fluorouracil in nasopharyngeal carcinoma CNE1 cells
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NFBD1/MDC1的敲低增强鼻咽癌CNE1细胞对顺铂或5-氟尿嘧啶的化疗敏感性
DOI：
10.1007/s11010-016-2739-5
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发表时间：
2016-06
影响因子：
4.3
通讯作者：
Hu, Guohua
中科院分区：
文献类型：
--
作者：
Kang, Houyong;Hong, Suling;Bu, Youquan;Hu, Guohua
鼻咽癌（NPC）是一种罕见但高度侵袭性的癌症，在中国南方和东南亚的中国南方血统人群中流行。放射治疗和以顺铂（CDDP）为基础的化疗是主要的治疗选择。不幸的是，鼻咽癌患者对同步放化疗的反应各不相同，许多病例对CDDP和放疗耐药。NFBD 1在细胞周期检查点激活和DNA损伤后的DNA修复中起作用。在这项研究中，我们确定了NFBD 1作为一个易处理的分子靶向鼻咽癌细胞的化疗增敏。使用慢病毒介导的短发夹RNA耗尽NPC CNE 1细胞系中的NFBD 1表达，并且使用MTS测定评估这些NFBD 1抑制的NPC细胞对治疗试剂CDDP和5-氟尿嘧啶（5-FU）的敏感性升高。流式细胞仪分析也表明，NFBD 1敲低导致CDDP或5-FU处理的CNE 1细胞中明显的凋亡诱导。此外，我们暗示NFBD 1参与CDDP或5-FU化疗后Rad 51和DNA-PKcs灶的形成。总之，NFBD 1基因敲低可通过抑制细胞生长和通过损伤DNA损伤修复促进细胞凋亡来提高鼻咽癌细胞的化疗敏感性，提示NFBD 1可作为鼻咽癌治疗的新靶点。
Nasopharyngeal carcinoma (NPC) is a rare but highly invasive cancer that is prevalent among people of southern Chinese ancestry in southern China and Southeast Asia. Radiotherapy and cisplatin (CDDP)-based chemotherapy are the main treatment options. Unfortunately, disease response to concurrent chemoradiotherapy varies among patients with NPC, and many cases are resistant to CDDP and radiotherapy. NFBD1 functions in cell cycle checkpoint activation and DNA repair following DNA damage. In this study, we identified the NFBD1 as a tractable molecular target to chemosensitize NPC cells. NFBD1 expression in NPC CNE1 cell lines was depleted using lentivirus-mediated short hairpin RNA, and the elevated sensitivity of these NFBD1-inhibited NPC cells to therapeutic reagent CDDP and 5-fluorouracil (5-FU) was evaluated using MTS assays. Flow cytometry analysis also showed that NFBD1 knockdown led to an obvious induction of apoptosis in CDDP- or 5-FU-treated CNE1 cells. Furthermore, we implicated the involvement of NFBD1 in Rad51 and DNA-PKcs foci formation following CDDP or 5-FU chemotherapy. In conclusion, NFBD1 knockdown improves the chemosensitivity of NPC cells by inhibiting cell growth and promoting apoptosis through the impairment of DNA damage repair, suggesting NFBD1 as a novel therapeutic target for NPC.
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影响因子：
9
作者：
通讯作者：
--
影响因子：
8
作者：
Wyatt, M. D.;Wilson, D. M., III
通讯作者：
Wilson, D. M., III
影响因子：
3.8
作者：
Banáth JP;Klokov D;MacPhail SH;Banuelos CA;Olive PL
通讯作者：
Olive PL
DOI：
10.1016/b978-0-12-814936-2.00002-x
发表时间：
2019
期刊：
Nasopharyngeal Carcinoma
影响因子：
--
作者：
M. Lung;Wei Dai;J. Ko
通讯作者：
M. Lung;Wei Dai;J. Ko
影响因子：
16
作者：
Lou, ZK;Minter-Dykhouse, K;Chen, JJ
通讯作者：
Chen, JJ