Phylogenetic characterization of six full-length HIV-1 subtype C molecular clones from three patients: identification of rare subtype C strains containing two NF-κB motifs in the long terminal repeat.
Phylogenetic characterization of six full-length HIV-1 subtype C molecular clones from three patients: identification of rare subtype C strains containing two NF-κB motifs in the long terminal repeat.
复制标题
来自三名患者的六个全长 HIV-1 C 亚型分子克隆的系统发育特征：鉴定在长末端重复中包含两个 NF-κB 基序的罕见 C 亚型毒株。
DOI：
10.1089/aid.2013.0275
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发表时间：
2014
影响因子：
1.5
通讯作者：
Prasad,VinayakaR
中科院分区：
文献类型：
--
作者：
Hanna,LukeElizabeth;Neogi,Ujjwal;Ranga,Udaykumar;Swaminathan,Soumya;Prasad,VinayakaR
Molecular surveillance is the backbone of HIV-1 vaccinology. Full-length HIV-1 sequences are useful tools that can provide a better understanding of the epidemiology in a given region. A limited number of full-length HIV-1 sequences are available from India, where >95% of the HIV infections are due to HIV-1 subtype C (HIV-1C), which is distinct from the prototype African HIV-1C. In this study, we sequenced six full-length clones isolated from three patients. Extensive phylogenetic analyses of the full-length viral sequences using bioinformatic tools identified a separate cluster of Indian strains, thus confirming the distinct phylogenetic identity of the Indian HIV-1C. Notably, the long terminal repeat (LTR) of two of the six molecular clones contained only two NF-κB binding sites. The sequences also displayed features characteristic of HIV-1C including a Tat dicysteine motif, a shortened Rev open reading frame, and a predicted CCR5 coreceptor tropism for gp120 of three of the proviral sequences.
