Characterization of phiCFP-1, a virulent bacteriophage specific for Citrobacter freundii
Characterization of phiCFP-1, a virulent bacteriophage specific for Citrobacter freundii
复制标题
phiCFP-1（一种弗氏柠檬酸杆菌特异的剧毒噬菌体）的表征
DOI：
10.1002/jmv.24401
复制
发表时间：
2016-05-01
影响因子：
12.7
通讯作者：
Yuan，Jing
中科院分区：
文献类型：
--
作者：
Zhao，Xiangna;Huang，Simo;Yuan，Jing
弗氏柠檬酸杆菌是一种革兰氏阴性细菌，可引起许多机会性感染。噬菌体phiCFP‐1被分离出来，并以其裂解多药耐药临床c的能力为特征。freundiistrain P10159。透射电镜显示，该噬菌体具有二十面体头部和短尾，为apodoviriae科成员。在单步生长实验中，phiCFP‐1表现出20分钟的月蚀期和每个细胞100个颗粒的爆发大小。基因组测序完成后，其基因组组装成一个环状分子。然而，基于基因组的内容和组织，它被归类为经典的T7相关噬菌体，已知这种噬菌体具有直接末端重复序列的线性基因组。利用本文建立的快速简便的方法，准确鉴定了38,625 bp的线性双链DNA，其中直接末端重复序列为229 bp。基因组包含43个假定的开放阅读框，没有tRNA基因。使用基于蛋白质组学的方法，从纯化的phiCFP - 1颗粒中鉴定了7个病毒蛋白和2个宿主蛋白。比较基因组学和重组分析显示，phiCFP‐1、phiYeO3‐12/vB_YenP_AP5（来自肠结肠炎耶尔森菌ao3）和phiSG‐JL2（来自肠沙门氏菌）具有密切的遗传亲缘关系。中华医学杂志，2016,33(5):391 - 391。©2015 Wiley期刊公司
Citrobacter freundii, a Gram‐negative bacterium, causes many opportunistic infections. Bacteriophage phiCFP‐1 was isolated and characterized by its ability to lyse the multidrug‐resistant clinicalC. freundiistrain P10159. Transmission electron microscopy showed that the phage has an icosahedral head and a short tail, making it aPodoviridaefamily member. In a single‐step growth experiment, phiCFP‐1 exhibited an eclipse period of 20 min and a burst size of 100 particles per cell. Its genome assembled as a circular molecule when genomic sequencing was completed. However, based on genome content and organization, it was categorized as a classic T7‐related phage, and such phages are known to have linear genomes with direct terminal repeats. With the quick and simple method established herein, the 38,625‐bp linear double‐stranded DNA with 229‐bp direct terminal repeats was accurately identified. The genome contained 43 putative open reading frames and no tRNA genes. Using a proteomics‐based approach, seven viral and two host proteins from purified phiCFP‐1 particles were identified. Comparative genomics and recombination analyzes revealed close genetic relatedness among phiCFP‐1, phiYeO3‐12/vB_YenP_AP5 (fromYersinia enterocoliticaO3), and phiSG‐JL2 (fromSalmonella enterica).J. Med. Virol. 88:895–905, 2016.© 2015 Wiley Periodicals, Inc.