c-Jun promotes neurite outgrowth and survival in PC12 cells
c-Jun promotes neurite outgrowth and survival in PC12 cells
复制标题
DOI：
10.1016/s0169-328x(00)00191-1
复制
发表时间：
2000-11-10
期刊：
影响因子：
--
通讯作者：
During, M
中科院分区：
文献类型：
--
作者：
Dragunow, M;Xu, RA;During, M
我们用含有c-dun cDNA转录盒的质粒转染PC 12细胞，研究了c-Jun在PC 12细胞中的功能。转染细胞表达高水平的c-Jun mRNA和蛋白，并表现出AP-1 DNA结合和基因激活的增加。过表达c-Jun的细胞表现出明显的神经突生长，但没有自发细胞死亡的证据。事实上，c-Jun过表达细胞对冈田酸诱导的细胞凋亡更具抵抗力。过程生长并不表示完全的神经元分化反应，因为当用全细胞膜片钳检查时，转染的PC 17细胞不显示动作电位。丝氨酸73上的c-Jun的磷酸化似乎对于这种神经突发芽效应是重要的，因为在该位点的诱变减少了发芽，而丝氨酸63突变体倾向于增加发芽。因此。在PC 17细胞中，c-Jun表达不诱导凋亡。而是用作神经突生长和神经元存活信号。(C)2000 Elsevier Science B. V.保留所有权利。
We investigated the function of c-Jun in PC12 cells by transfecting them with a plasmid containing a c-dun cDNA transcription cassette. Transfected cells expressed high levels of c-Jun mRNA and protein and demonstrated an increase in both AP-1 DNA binding and gene activation. The c-Jun over-expressing cells showed marked neurite outgrowth but no evidence of spontaneous cell death. In fact, c-Jun over-expressing cells were more resistant to okadaic acid-induced apoptosis. The process outgrowth was not indicative of a full neuronal differentiation response as the transfected PC17 cells did not display action potentials when examined with whole-cell patch-clamping. The phosphorylation of c-Jun on serine 73 appears to be important for this neurite sprouting effect as mutagenesis at this site reduced sprouting whereas a serine 63 mutant tended to increase sprouting. Thus. in PC17 cells c-Jun expression does not induce apoptosis. but rather functions as a neurite outgrowth and neuronal survival signal. (C) 2000 Elsevier Science B.V. All rights reserved.