Syringe‐dispensed omega‐3 lipid injectable emulsions should be stored under airtight refrigeration: A proposal for the efficient supply of unapproved precious lipid resources
Syringe‐dispensed omega‐3 lipid injectable emulsions should be stored under airtight refrigeration: A proposal for the efficient supply of unapproved precious lipid resources
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注射器分配的 omega-3 脂质注射乳剂应密闭冷藏储存：关于有效供应未经批准的珍贵脂质资源的建议
DOI：
10.1002/jpen.2443
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发表时间：
2022
影响因子：
3.4
通讯作者：
Ibara Satoshi
中科院分区：
文献类型：
--
作者：
Setoguchi Makoto;Muto Mitsuru;Ohata Tomonori;Fukuoka Ryuichi;Ikeda Hirohito;Aki Hatsumi;Haraguchi Masaomi;Hanjo Shota;Arima Junko;Ibara Satoshi
背景鱼油脂质注射乳剂(FO-ILE)和混合油脂质注射乳剂(MO-ILE)都是肠外营养的关键成分，需要进口到日本，开封后很容易氧化。考虑到患有肠衰竭（IF）的婴儿和儿童每日分配的这些脂质的量较小，本研究的目的是确定最佳的储存方法。方法按照以下方式在聚丙烯注射器中制备脂质：空气密封和光保护、仅空气密封、仅光保护和无盖。样品在 4°C 或 26°C 下保存 14 天。通过测量丙二醛 (MDA) 浓度和 pH 值并将其与开封后立即测量的值进行比较来评估氧化降解程度。结果对于 FO-ILE，无论是否进行光保护（+0.45μM，P= 1.0）或无光保护（+0.52μM，P= 1.0），气密样品中 14 天的 MDA 浓度增加均不显着。这种趋势在 4°C 时比 26°C 时更明显 (P< 0.01)。 4°C 时 pH 值最大下降为 0.08。无论是否进行光保护（+0.36μM，P= 0.11）或无光保护（+0.33μM，P= 0.76），MO-ILE 在气密样品中表现出 MDA 浓度连续 14 天无显着增加。这种趋势在 4°C 时比 26°C 时更明显 (P< 0.01)。 4°C 时 pH 值最大下降为 0.12。对于大豆油脂质注射乳剂，趋势相似，没有明显变质。结论注射器分配的 FO-ILE 和 MO-ILE 在密闭冷藏下储存 14 天仍未变质。当为 IF 婴儿提供这些昂贵的资源时，我们的结果被认为具有临床价值。
BackgroundBoth fish‐oil lipid injectable emulsion (FO‐ILE) and mixed‐oil lipid injectable emulsion (MO‐ILE) are key components of parenteral nutrition and require importation into Japan, and they are easily oxidized after opening. Given the small daily volumes of these lipids dispensed in infants and children with intestinal failure (IF), the purpose of the study was to identify the optimal storage method.MethodsLipids were prepared in polypropylene syringes in the following manner: air‐sealing and photoprotection, air‐sealing only, photoprotection only, and uncovered. Samples were stored for 14 days at 4°C or 26°C. The degree of oxidative degradation was evaluated by measuring malondialdehyde (MDA) concentration and pH and comparing them to the values measured immediately after opening.ResultsFor FO‐ILE, the increase in MDA concentration for 14 days was insignificant in air‐sealed samples, regardless of photoprotection (+0.45 μM,P= 1.0) or no photoprotection (+0.52 μM,P= 1.0). This trend was more pronounced at 4°C than at 26°C (P< 0.01). The maximum pH decrease was 0.08 at 4°C. MO‐ILE exhibited an insignificant increase in MDA concentration for 14 days with air‐sealed samples, regardless of photoprotection (+0.36 μM,P= 0.11) or no photoprotection (+0.33 μM,P= 0.76). This trend was more pronounced at 4°C than at 26°C (P< 0.01). The maximum pH decrease was 0.12 at 4°C. For soybean‐oil lipid injectable emulsion, the trend was similar with no considerable deterioration.ConclusionSyringe‐dispensed FO‐ILE and MO‐ILE stored under airtight refrigeration remained undeteriorated for 14 days. Our results are considered clinically valuable when supplying these expensive resources for infants with IF.