Resistance to carbosulfan in Anopheles gambiae from Ivory Coast, based on reduced sensitivity of acetylcholinesterase
Resistance to carbosulfan in Anopheles gambiae from Ivory Coast, based on reduced sensitivity of acetylcholinesterase
复制标题
DOI：
10.1046/j.1365-2915.2003.00406.x
复制
发表时间：
2003-03-01
影响因子：
1.9
通讯作者：
Chandre, F
中科院分区：
文献类型：
--
作者：
N'Guessan, R;Darriet, F;Chandre, F
在来自象牙海岸布瓦凯附近两个生态对比不同地区的疟疾媒介按蚊冈比亚按蚊(双翅目：蚊科)的野外种群中检测到对氨基甲酸酯杀虫剂丁硫丹的抗药性：M‘be农村地区以M型为主。对拟除虫菊酯敏感的冈比亚；郊区的姚科菲克罗，主要是S形式的AN。冈比亚对拟除虫菊酯高度抗性(96%kdr)。通过对敏感AN的生物测定，确定了0.4%丁硫克百威的鉴别浓度(即LC_(100)的两倍)。冈比亚基苏木品系。在诊断剂量(0.4%丁硫克百威，作用1h)暴露后，雌蚊死亡率下降。暴露后24 h，冈比亚成虫(取自稻田的幼虫)分别是M‘be和Yaokoffikro成虫的62%和29%。用200 mg/m(2)丁硫克百威浸泡蚊帐3min，死亡率为M‘be的88%，姚科菲克罗的12.2%。在每个病例中，未经治疗的对照死亡率都微乎其微。生化分析检测可能的抗性机制(S)表明，在按蚊种群中存在不敏感的AChE。根据生物测定结果，在两个地方都有冈比亚，在姚科菲克罗的S形式比在M‘be的M形式更普遍。我们的研究表明，有必要监测人群对氨基甲酸酯的耐药性。非洲冈比亚复合体，以确定其传播情况，并预测如果使用这些杀虫剂，病媒控制将失败。
Resistance to carbosulfan, a carbamate insecticide, was detected in field populations of the malaria vector mosquito Anopheles gambiae Giles (Diptera: Culicidae) from two ecologically contrasted localities near Bouake, Ivory Coast: rural M'be with predominantly M form of An. gambiae susceptible to pyrethroids; suburban Yaokoffikro with predominantly S form of An. gambiae highly resistant to pyrethroids (96% kdr). The discriminating concentration of 0.4% carbosulfan (i.e. double the LC100) was determined from bioassays with the susceptible An. gambiae Kisumu strain. Following exposure to the diagnostic dosage (0.4% carbosulfan for 1 h), mortality rates of female An. gambiae adults (reared from larvae collected from ricefields) were 62% and 29% of those from M'be and Yaokoffikro, respectively, 24 h post-exposure. Exposure for 3 min to netting impregnated with the operational dosage of carbosulfan 200 mg/m(2) gave mortality rates of 88% of those from M'be and only 12.2% for Yaokoffikro. In each case the control untreated mortality rate was insignificant. Biochemical assays to detect possible resistance mechanism(s) revealed the presence of insensitive AChE in populations of An. gambiae at both localities, more prevalent in the S form at Yaokoffikro than in M form at M'be, as expected from bioassays results. Our study demonstrates the need to monitor carbamate resistance among populations of the An. gambiae complex in Africa, to determine its spread and anticipate vector control failure if these insecticides are employed.