Synergy of 1,25-dihydroxyvitamin D3 and carboplatin in growth suppression of SKOV-3 cells.
Synergy of 1,25-dihydroxyvitamin D3 and carboplatin in growth suppression of SKOV-3 cells.
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1,25-二羟基维生素 D3 和卡铂在抑制 SKOV-3 细胞生长中的协同作用
DOI：
10.3892/ol.2014.2307
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发表时间：
2014-09
期刊：
影响因子：
2.9
通讯作者：
Li B
中科院分区：
文献类型：
--
作者：
Zhang Z;Zhang H;Hu Z;Wang P;Wan J;Li B
1α,25-Dihydroxyvitamin D3 [1,25(OH)2D3] has been demonstrated to inhibit the growth of cancer cells. However, carboplatin is the most widely used chemotherapeutic agent to treat cancer. We hypothesized that vitamin D may enhance the antiproliferative effects of carboplatin, and tested this hypothesis in ovarian cancer SKOV-3 cells treated with carboplatin and 1,25(OH)2D3. Cell viability was determined by Cell Counting Kit-8, while cell cycle distribution, apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were analyzed by flow cytometry. In these experiments, 1,25(OH)2D3 and carboplatin each provided dose-dependent suppression of SKOV-3 growth, and synergy was demonstrated between 10 nM 1,25(OH)2D3 and carboplatin. The proportion of cells in G0/G1 phase was markedly reduced by the drug combination, while the proportion of cells in G2/M phase was increased. Apoptosis did not increase in ovarian cancer cells treated with 10 nM 1,25(OH)2D3 alone; however, 1,25(OH)2D3 evidently enhanced carboplatin-induced apoptosis. Similarly, ROS production was evidently higher and MMP was lower in cells treated with the two drugs than in those treated with each drug alone. The results suggested that 1,25(OH)2D3 suppresses SKOV-3 growth and enhances the antiproliferative effect of carboplatin. The drugs function synergistically by inducing cell cycle arrest, increasing apoptosis and ROS production, and reducing MMP.
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影响因子：
4.8
作者：
DOKOH, S;DONALDSON, CA;HAUSSLER, MR
通讯作者：
HAUSSLER, MR
DOI：
10.1016/0165-3806(95)00204-9
发表时间：
1996-03-29
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
Johnson, JA;Grande, JP;Kumar, R
通讯作者：
Kumar, R
影响因子：
3
作者：
Cheng, Timothy C.;Manorek, Gerald;Howell, Stephen B.
通讯作者：
Howell, Stephen B.
影响因子：
6.4
作者：
Bao, Bo-Ying;Ting, Huei-Ju;Lee, Yi-Fen
通讯作者：
Lee, Yi-Fen
影响因子：
4.7
作者：
Markman, M;Webster, K;Belinson, J
通讯作者：
Belinson, J