EPR studies on the superoxide-scavenging capacity of the nutraceutical resveratrol
EPR studies on the superoxide-scavenging capacity of the nutraceutical resveratrol
复制标题
DOI：
10.1007/s11010-008-9756-y
复制
发表时间：
2008-06-01
影响因子：
4.3
通讯作者：
Misra, Hara P.
中科院分区：
文献类型：
--
作者：
Jia, Zhenquan;Zhu, Hong;Misra, Hara P.
白藜芦醇（3,4'，5-三羟基二苯乙烯）是一种多酚类化合物，存在于桑葚、葡萄和红酒中，由于其潜在的抗氧化活性，对各种退行性疾病具有明显的保护作用，因此受到了相当大的关注。然而，文献中缺乏白藜芦醇清除超氧化物能力的直接证据。在本研究中，利用电子顺磁共振波谱结合5-（二氧基磷酰）-5-甲基吡啶- n -氧化物(DEPMPO)-自旋捕获技术来测定白藜芦醇清除超氧化物钾和黄嘌呤氧化酶/黄嘌呤体系产生的超氧阴离子的能力。我们在这里首次证明了白藜芦醇的存在导致超氧化物钾和黄嘌呤氧化酶/黄嘌呤系统中depmpo -超氧化物加合物（DEPMPO-OOH）的形成减少，表明白藜芦醇可以直接清除超氧化物阴离子。然而，在黄嘌呤氧化酶/黄嘌呤系统中，DEPMPO-OOH的抑制作用比在钾超氧化物系统中观察到的要强得多。结果表明，白藜芦醇还能直接抑制黄嘌呤氧化酶活性。综上所述，本研究报道的白藜芦醇在直接清除超氧化物和通过黄嘌呤氧化酶抑制超氧化物生成方面的双重作用，可能至少部分解释了该化合物在各种疾病过程中对氧化损伤的保护作用。
Resveratrol (3,4',5-trihydroxystilbene), a polyphenolic compound found in mulberries, grapes, and red wine, has received considerable attention because of its apparent protective effects against various degenerative diseases due to its potential antioxidant activities. However, direct evidence for the superoxide-scavenging capacity of resveratrol is lacking in literature. In this study, electron paramagnetic resonance spectroscopy in combination with 5-(diethoxyphosphoryl)-5-methylpyrroline-N-oxide (DEPMPO)-spin trapping technique was utilized to determine the ability of resveratrol in scavenging superoxide anions generated from both potassium superoxide and the xanthine oxidase/xanthine system. We have demonstrated here for the first time that the presence of resveratrol resulted in decreased formation of DEPMPO-superoxide adduct (DEPMPO-OOH) in both the potassium superoxide and xanthine oxidase/xanthine systems, indicating that resveratrol could directly scavenge superoxide anions. The inhibition of DEPMPO-OOH in the xanthine oxidase/xanthine system, however, was found to be much potent as compared to that observed in potassium superoxide system. It was further shown that resveratrol could also directly inhibit xanthine oxidase activity as assessed by oxygen consumption and formation of uric acid. Taken together, the dual role of resveratrol in directly scavenging superoxide and inhibiting its generation via xanthine oxidase reported in this study may explain, at least in part, the protective role of this compound against oxidative injury in various disease processes.