Phenazine enables the anaerobic respiration of Pseudomonas aeruginosa via electron transfer with a polarised graphite electrode
Phenazine enables the anaerobic respiration of Pseudomonas aeruginosa via electron transfer with a polarised graphite electrode
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吩嗪通过极化石墨电极的电子转移实现铜绿假单胞菌的无氧呼吸
DOI：
10.1016/j.ibiod.2018.11.002
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发表时间：
2019-02-01
影响因子：
4.8
通讯作者：
Hou, Baorong
中科院分区：
文献类型：
--
作者：
Guan, Fang;Yuan, Xuechan;Hou, Baorong
铜绿假单胞菌PAO1广泛分布于淡水、土壤和海洋环境中，可作为电化学活性菌在生物电化学系统中进行生物转化。吩嗪是PAO1产生的一种活性氧化还原化合物，作为一种替代呼吸色素，可能对其生产者有益。在这里，我们证明了吩嗪促进PAO1的电子转移到电位电极，从而促进铜绿假单胞菌PAO1的厌氧生长。在磷酸盐缓冲盐水培养基生物反应器中，研究了4种厌氧条件下铜绿假单胞菌PAO 1的生长和胞外电子传递：有平衡电位但无吩嗪、有吩嗪但无平衡电位、有吩嗪和平衡电位以及无吩嗪但无平衡电位。结果表明，无极化电极或无吩嗪的厌氧条件不支持铜绿假单胞菌PAO 1在滤膜上形成殖民地。吩嗪增强了铜绿假单胞菌PAO1与电极间的电子传递。这些发现为了解铜绿假单胞菌PAO1的无氧呼吸提供了一个新的视角。
Pseudomonas aeruginosa PAO1 is widely distributed in fresh water, soil, and marine environments and can act as electrochemically active bacteria for bioconversion in bioelectrochemical systems. Phenazine, an active redox compound produced by PAO1, could be beneficial for its producers as an alternative respiratory pigment. Here, we demonstrated that phenazine promoted electron transfer of PAO1 to poised-potential electrodes, thus facilitating the anaerobic growth of P. aeruginosa PAO1. The growth and extracellular electron transfer of P. aeruginosa PAO1 were studied under four anaerobic conditions: with poised potential but without phenazine, with phenazine but without poised potential, with both phenazine and poised potential, and without phenazine and without poised potential in a phosphate-buffered saline medium bioreactor. The results showed that anaerobic conditions, without polarized electrode or without phenazine, did not support the colony formation of P. aeruglnoa PAO1 on filters. Phenazine enhanced the electron transport between P. aeruginosa PAO1 and poised-potential electrode. These findings provided an insight into understanding the anaerobic respiration of P. aeruginosa PAO1 with poised-potential electrodes.