Genomic organization and context of a trypanosome variant surface glycoprotein gene family.
Genomic organization and context of a trypanosome variant surface glycoprotein gene family.
复制标题
锥虫变异表面糖蛋白基因家族的基因组组织和背景。
DOI：
10.1016/0022-2836(92)90097-4
复制
发表时间：
1992
影响因子：
5.6
通讯作者：
Boothroyd,JC
中科院分区：
文献类型：
--
作者：
Beals,TP;Boothroyd,JC
我们定义了布氏锥虫布氏锥虫表面糖蛋白(VSG)基因家族的基因组结构和基因组环境。这个基因家族在基因组中既不是重复的，也不是紧密连锁的，也不位于小染色体或中等大小的染色体上。两个分散的重复序列元件Rime-Ingi和上游重复序列与该基因家族的成员相连；然而，上游重复序列仅与基本拷贝紧密相连。在其他分离物中经常出现。布鲁氏杆菌基因家族具有保守性，但有一定的变异；在这些分离株中发现的限制性片段长度多态性表明，VSG基因可能偶尔是二倍体。提出了一个既能解释分散的VSG基因家族的产生，又能解释VSG基因的株间变异性的模型。
We have defined the genomic organization and genomic context of aTrypanosoma brucei bruceigene family encoding variant surface glycoproteins (VSGs). This gene family is neither tandemly repeated nor closely linked in the genome, and is not located on small or intermediate size chromosomes. Two dispersed repeated sequence elements, RIME-ingi and the upstream repeat sequence, are linked to members of this gene family; however, the upstream repeat sequences are closely linked only to the basic copy. In other isolates ofT. b. bruceithis gene family appears conserved with some variation; a restriction fragment length polymorphism found among these isolates suggests the hypothesis that VSG genes may occasionally be diploid. A model accounting for both the generation of dispersed families of VSG genes, and for the interstrain variability of VSG genes, is proposed.