Expression-of mRNAs for interleukin-4, interleukin-6 and their receptors in porcine corpus luteum during the estrous cycle
Expression-of mRNAs for interleukin-4, interleukin-6 and their receptors in porcine corpus luteum during the estrous cycle
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DOI：
10.1016/j.domaniend.2005.11.001
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发表时间：
2006-10-01
影响因子：
2.1
通讯作者：
Okuda, Kiyoshi
中科院分区：
文献类型：
--
作者：
Sakumoto, Ryosuke;Komatsu, Tokushi;Okuda, Kiyoshi
越来越多的证据表明，炎性细胞因子在许多物种中调节黄体功能。本研究的目的是确定IL-4和IL-6是否在猪CL中表达，以及这些细胞因子是否影响猪黄体类固醇激素的生成。测定了中国梅山猪发情周期CL中IL-4、1L-6及其特异性受体的基因表达。此外，还观察了这些细胞因子对黄体细胞分泌孕酮(P-4)、17β雌二醇(E-2)和前列腺素(PG)F2α的影响。IL-4和IL-6mRNAs在黄体各期均有表达。此外，IL-4和IL-6受体的mRNAs在整个发情周期的CL中都有明显表达。实时荧光定量聚合酶链式反应分析显示，消退型CL(排卵后19~21天)IL-6受体(IL-6R)mRNA的表达高于其他时期(P<0.01)。IL-6(1-100 ng/ml)可抑制黄体细胞分泌P-4和E-2(P<；0.05)。IL-4(1~100 ng/ml)对P-4分泌无明显影响，但对E-2分泌有抑制作用(P<；0.05)。IL-4和IL-6对细胞分泌PGF2α均无影响。这些结果表明，IL-4和IL-6在猪CL局部产生，它们通过其特异性受体抑制黄体细胞类固醇的产生。综上所述，IL-4和IL-6可能在整个发情周期中对猪CL功能起调控作用。(C)2005 Elsevier Inc.保留所有权利。
There is increasing evidence that inflammatory cytokines regulate corpus luteum CL) function in many species. The purpose of the present study was to determine whether interleukin (IL)-4 and IL-6 are expressed in the porcine CL, and whether these cytokines influence porcine luteal steroidogenesis. The gene expressions of IL-4, 1L-6 and their specific receptors were determined in the CL of Chinese Meishan pigs during the estrous cycle. Moreover, the effects of these cytokines on progesterone (P-4), estradiol-17 beta (E-2) and prostaglandin (PG) F2 alpha secretion by cultured luteal cells were investigated. IL-4 and IL-6 mRNAs were detected in the CL at all luteal stages. Furthermore, mRNAs of the receptors for IL-4 and IL-6 were clearly expressed in the CL throughout the estrous cycle. Real-time PCR analysis revealed that IL-6 receptor (IL-6R) mRNA expression was higher in the regressed CL (days 19-21 after ovulation) than in the CL at other stages (P < 0.01). Exposure of cultured luteal cells obtained from mid-stage CL (days 8-11) to IL-6 (1-100 ng/ml), it inhibited P-4 and E-2 secretion by the cells (P < 0.05). Although IL-4 (1-100 ng/ml) did not significantly alter P-4 secretion, it inhibited E-2 secretion by the cells (P < 0.05). Neither IL-4 nor IL-6 had any effect on PGF2 alpha secretion by the cells. These results suggest that IL-4 and IL-6 are locally produced in the porcine CL, and that they inhibit steroid production from luteal cells via their specific receptors. Collectively, both IL-4 and IL-6 may play roles in regulating porcine CL function throughout the estrous cycle. (c) 2005 Elsevier Inc. All rights reserved.