Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Nonradioactive Oligo Probes.
Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Nonradioactive Oligo Probes.
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DOI：
10.1007/978-1-4939-7237-1_3
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发表时间：
2017
影响因子：
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通讯作者：
Tianyun Long;R. Lu
中科院分区：
文献类型：
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作者：
Tianyun Long;R. Lu
北方印迹分析已被广泛用作检测和表征特定RNA分子的工具。当与放射性探针结合时，北方印迹允许痕量小RNA分子的稳健检测和表征。在这里，我们描述了病毒衍生的小干扰RNA（vsiRNA）在C中的检测和大小表征。用非放射性DNA寡核苷酸探针进行北方杂交。我们的方案不仅允许初级vsiRNA的检测和表征，而且还允许次级vsiRNA的检测和表征，次级vsiRNA是一类具有不同迁移模式的单链vsiRNA，并且可以容易地适用于检测其他生物体中的vsiRNA。
Northern blot analysis has been widely used as a tool for detection and characterization of specific RNA molecules. When coupled with radioactive probe northern blot allows for robust detection and characterization of small RNA molecules of trace amount. Here, we describe the detection and size characterization of virus-derived small interfering RNAs (vsiRNAs) inC. elegansusing nonradioactive DNA oligo probes in northern blotting. Our protocol allows for the detection and characterization of not only primary vsiRNAs but also secondary vsiRNAs, a class of single-stranded vsiRNAs that has distinct migration pattern, and can be easily adapted to the detection of vsiRNAs in other organisms.