Novel heme-binding component in the serum of the channel catfish (Ictalurus punctatus).
Novel heme-binding component in the serum of the channel catfish (Ictalurus punctatus).
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斑点叉尾鮰（Ictalurus punctatus）血清中的新型血红素结合成分。
DOI：
10.1007/bf01079699
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Byers,BR
中科院分区：
文献类型：
--
作者：
Massad,G;Arceneaux,JE;Byers,BR
对斑点叉尾（Ictalurus punctatus）的血清进行了血红素和血红蛋白结合蛋白的检测。电泳迁移率阻滞试验未能检测到血红蛋白结合材料类似哺乳动物触珠蛋白，然而，血红素结合成分（以前没有描述）被确定在鲶鱼血清。血红素结合组分通过凝胶过滤色谱法纯化;电泳分析表明它由分子量约为115和98 kDa的两个多肽亚基组成。该组成与血红素结合蛋白（已知的哺乳动物血红素结合血清蛋白）不一致。虽然它不是完全饱和的血红素，鲶鱼成分中含有可检测的血红素在正常血清。当与结合材料复合时，血红素被革兰氏阴性气单胞菌属的细菌分离株用作铁源;其他细菌使用复合物的能力未进行测试。鲶鱼血红素结合血清蛋白的生理功能目前尚不清楚。
The serum of the channel catfish (Ictalurus punctatus) was examined for heme- and hemoglobin-binding proteins. Electrophoretic mobility retardation assays failed to detect a hemoglobin-binding material similar to mammalian haptoglobin; however, a heme-binding component (not previously described) was identified in catfish seru. The heme-binding component was purified by gel filtration chromatography; electrophoretic analyses suggested it to be composed of two polypeptide subunits of molecular masses about 115 and 98 kDa. This composition is inconsistent with hemopexin, the known heme-binding serum protein of mammals. Although it was not fully saturated with heme, the catfish component contained detectable heme in normal sera. When complexed by the binding material, heme was used as an iron source by isolates of the bacterial Gram-negative genusAeromonas; the capacity of other bacteria to use the complex was not tested. The physiological function of the catfish heme-binding serum protein is presently not clear.