Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4
Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4
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DOI：
10.1099/vir.0.2008/000380-0
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发表时间：
2008-08-01
影响因子：
3.8
通讯作者：
Yuen, Kwok-Yung
中科院分区：
文献类型：
--
作者：
Lau, Susanna K. P.;Woo, Patrick C. Y.;Yuen, Kwok-Yung
人细小病毒4型（Human parvovirus 4，PARV 4）是最近发现的一种仅存在于人血浆和肝组织中的细小病毒，被认为在遗传学上与其他细小病毒不同。在此，我们报告了两种新的细小病毒密切相关的PARV 4，猪和牛的病毒（PHoV），猪和牛的病毒（BHoV），从猪和牛的样本中发现。还分析了它们的近全长序列。用PCR方法在44.4%（148/ 333）的猪样品（包括淋巴结、肝脏、血清、鼻咽和粪便样品）、13%（4/32）的牛脾样品和2%（7/362）的人血清样品中检测到PARV 4样病毒。鉴定了三种不同的细小病毒，包括两种新的细小病毒，分别来自猪和牛样品的PHoV和BHoV以及来自人的PARV 4。对7株PHoV、3株BHoV和1株PARV 4的基因组序列分析表明，这两种动物细小病毒与PARV 4最相似，核苷酸同源性为61.5- 63%，并与PARV 4（HHoV）一起在细小病毒科中形成了一个独特的簇。这三种细小病毒与其他细小病毒的不同之处还在于它们相对较大的预测VP 1蛋白和存在一个小的独特的保守推定蛋白。基于这些结果，我们提出了一个单独的属，Hokovirus，来描述这三种细小病毒。猪繁殖与呼吸综合征病毒的共检测，与最近在中国的猪“高热”疾病爆发，从我们的猪样本值得进一步调查。
Human parvovirus 4 (PARV4), a recently discovered parvovirus found exclusively in human plasma and liver tissue, was considered phylogenetically distinct from other parvoviruses. Here, we report the discovery of two novel parvoviruses closely related to PARV4, porcine hokovirus (PHoV) and bovine hokovirus (BHoV), from porcine and bovine samples in Hong Kong. Their nearly full-length sequences were also analysed. PARV4-like viruses were detected by PCR among 44.4% (148/ 333) of porcine samples (including lymph nodes, liver, serum, nasopharyngeal and faecal samples), 13 % (4/32) of bovine spleen samples and 2 % (7/362) of human serum samples that were sent for human immunodeficiency virus and hepatitis C virus antibody tests. Three distinct parvoviruses were identified, including two novel parvoviruses, PHoV and BHoV, from porcine and bovine samples and PARV4 from humans, respectively. Analysis of genome sequences from seven PHoV strains, from three BHoV strains and from one PARV4 strain showed that the two animal parvoviruses were most similar to PARV4 with 61.5-63 % nt identities and, together with PARV4 (HHoV), formed a distinct cluster within the family Parvoviridae. The three parvoviruses also differed from other parvoviruses by their relatively large predicted VP1 protein and the presence of a small unique conserved putative protein. Based on these results, we propose a separate genus, Hokovirus, to describe these three parvoviruses. The co-cletection of porcine reproductive and respiratory syndrome virus, the agent associated with the recent 'high fever' disease outbreaks in pigs in China, from our porcine samples warrants further investigation.