Enantioselective esterification of ibuprofen by a novel thermophilic Biocatalyst: APE1547
Enantioselective esterification of ibuprofen by a novel thermophilic Biocatalyst: APE1547
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DOI：
10.1007/s12257-011-0007-9
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发表时间：
2011-10
影响因子：
3.2
通讯作者：
Dantong Zhao;Er-na Xun;Jiaxin Wang;Ren Wang;Xiao-Fei Wei;Lei Wang;Zhi Wang
中科院分区：
文献类型：
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作者：
Dantong Zhao;Er-na Xun;Jiaxin Wang;Ren Wang;Xiao-Fei Wei;Lei Wang;Zhi Wang
在有机溶剂中，利用古菌Aeropyrum pernixK 1产生的一种新型嗜热酯酶（APE 1547）催化布洛芬的对映选择性酯化反应。考察了酰基受体、底物配比、有机溶剂、温度、水活度等因素对反应的影响。在最佳条件下，酶活为216.5 μmol/g/h，对映体选择性为38.1。向反应混合物中加入硅藻土以除去酯化反应中产生的水。反应在约96小时内达到平衡，转化率为57%，得到99%（ee）的未反应的（S）-布洛芬。
The enantioselective esterification of ibuprofen catalyzed by a novel thermophilic esterase (APE1547) from the archaeonAeropyrum pernixK1 was successfully conducted in organic solvents. The effects of acyl acceptor, substrate ratio, organic solvent, temperature, and water activity were investigated. Under optimum conditions, the highest enantioselectivity (E= 38.1) was obtained with a higher enzyme activity (216.5 μmol/g/h). Celites were added into the reaction mixture to remove the water produced in the esterification. The reaction achieved its equilibrium in approximately 96 h with a conversion of 57 and 99% (ee) of the un-reacted (S)-ibuprofen obtained.