COUPLING IN RAT HIPPOCAMPAL SLICES - DYE TRANSFER BETWEEN CA1 PYRAMIDAL CELLS
COUPLING IN RAT HIPPOCAMPAL SLICES - DYE TRANSFER BETWEEN CA1 PYRAMIDAL CELLS
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DOI：
10.1016/0361-9230(82)90048-x
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发表时间：
1982-01-01
影响因子：
3.8
通讯作者：
DUDEK, FE
中科院分区：
文献类型：
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作者：
ANDREW, RD;TAYLOR, CP;DUDEK, FE
将Lucifer Yellow-CH （LY）注射到大鼠海马CA1锥体细胞中，研究神经元之间的染料转移，作为这些细胞电张力耦合的证据。进行了广泛的控制程序，大大减少了无意的染色。注射层状锥体后，半数以上的神经元染色偶联。即使在峰值振幅为0.10%时，染料偶联也会发生。70 mV贯穿贯穿贯穿，并且在化学突触被含有Mn2+的低Ca2+溶液阻断后仍然存在。染料偶联细胞的胞体多位于35亩内。M（后固定）的注射细胞，并没有表现出偏好的方向。快速预电位和染料偶联是独立发生的。颈上神经节的神经元切片注射后，未见染料偶联。在放射状层中注入LY的树突内也产生了ca1锥体细胞之间的染料偶联，尽管染料偶联频率较低。在辐射层内，细胞外抛射和中间神经元细胞内注射均与多重染色无关。将LY注射到单个CA1锥体细胞的体细胞或树突中，通常会导致多次染色，并且在许多集合中，体细胞间隔很好。对照实验表明这种染料转移不是通过细胞外途径进行的。这表明一些CA1细胞是电致偶联的。需要进一步的电生理和形态学研究来解决用于评估海马体中偶联量的各种技术之间的差异。
Intracellular injections of Lucifer Yellow-CH (LY) into CA1 pyramidal cells were made in rat hippocampal slices to study dye transfer between neurons as evidence that these cells are electrotonically coupled. Extensive ntrol procedures were performed which substantially reduced inadvertent staining. Over half of the neurons were dye-coupled after injections in stratus pyramidale. Dye coupling occurred even when spike amplitudes were .gtoreq. 70 mV throughout the impalement and was still present after chemical synapses were blocked with a low Ca2+ solution containing Mn2+. Somata of dye-coupled cells were usually located within 35 .mu.m (post-fixation) of the injected cell and showed no preferred orientation. Fast prepotentials and dye coupling occurred independently. Neurons in superior cervical ganglia, which were sliced and injected using similar procedures, showed no dye coupling. Intradendritic injections of LY in stratum radiatum also yielded dye coupling between CA 1 pyramidal cells, although the dye coupling was less frequent. Within stratum radiatum, neither extracellular ejections nor intracellular injections of interneurons were associated with multiple staining. Injection of LY into the soma or dendrite of a single CA1 pyramidal cell often resulted in multiple staining, and in many ensembles the somata were well spaced. Control experiments suggested that such dye transfer is not by an extracellular route. This implies that some CA1 cells are electrotonically coupled. Further electrophysiological and morphological studies are required to resolve the discrepancies among various techniques used to evaluate the amount of coupling in the hippocampus.