Exploring the multilevel hazards of thiamethoxam using Drosophila melanogaster
Exploring the multilevel hazards of thiamethoxam using Drosophila melanogaster
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使用黑腹果蝇探索噻虫嗪的多层次危害
DOI：
10.1016/j.jhazmat.2019.121419
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发表时间：
2020-02-15
影响因子：
13.6
通讯作者：
Wang, Xing
中科院分区：
文献类型：
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作者：
Li, Xiaoqin;Liu, Jinyue;Wang, Xing
噻虫嗪（THIA）是一种广泛使用的农药。然而，其对昆虫生长发育的影响尚不清楚。在此，鉴定了THIA的致死浓度50（LC 50）（对于成虫为3.13 μ g/mL，对于三龄幼虫为20.25 μ g/mL）。THIA（1/3LC_（50））可延长果蝇生长发育所需时间，降低果蝇的生殖力、化蛹率和羽化率，延长寿命。复眼的均匀结构被打乱。它还引发DNA损伤，降低脂肪体细胞和血细胞的活力。此外，RNA测序结果显示，THIA诱导的差异表达基因主要与角质层发育、免疫功能有关。角质层蛋白（Lcp 65 Ag 3、Cpr 65 Ax 1）、血细胞增殖（RyR）和免疫应答（IM 4）中涉及的基因被显著诱导。与脂质代谢（sxe 2）、寿命（Atg 7和NalZ）、蛹发育（48，Blimp-1）、雌性生育力（Ddc）、雄性交配行为（pie）、神经视网膜发育（Nnad）相关的基因显著下调。这些发现为进一步研究提供了基础，以充分评估接触类烟碱农药的危害。
Thiamethoxam (THIA) is a widely used pesticide. However, its effects on the growth and development of insects remain unclear. Herein, the lethal concentration 50 (LC50) of THIA (3.13 mu g/mL for adults, 20.25 mu g/mL for third-instar larvae) were identified. THIA (1/3 LC50) prolonged the time required for growth and development, and decreased the fecundity, the rates of pupation and eclosion, and lifespan of Drosophila. The uniform architecture of the compound eyes was disturbed. It also triggered DNA damage, and reduced the viability of fat body cells and hemocytes. Moreover, RNA-sequencing showed that differentially expressed genes in response to THIA were mainly related to stratum cometun development, immune function. Genes involved in stratum comeum proteins (Lcp65Ag3, Cpr65Ax1), hemocyte proliferation (RyR), and immune responses (IM4) were significantly induced. Genes associated with lipid metabolism (sxe2), lifespan (Atg7 and NalZ), pupa development (48, Blimp-1), female fertility (Ddc), male mating behavior (pie), neural retina development (Nnad), was significantly downregulated. These findings provide a basis for further research to fully assess the hazards of exposure to neonicotinoid pesticides.