Activity-regulated cytoskeleton-associated protein Arc is targeted to dendrites and coexpressed with mu-opioid receptors in postnatal rat caudate-putamen nucleus.
Activity-regulated cytoskeleton-associated protein Arc is targeted to dendrites and coexpressed with mu-opioid receptors in postnatal rat caudate-putamen nucleus.
复制标题
活性调节细胞骨架相关蛋白 Arc 靶向树突，并与出生后大鼠尾壳核中的 mu-阿片受体共表达。
DOI：
10.1002/jnr.20173
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Pickel,VirginiaM
中科院分区：
文献类型：
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作者：
Wang,Hong;Pickel,VirginiaM
树突状表达的活性调节的细胞凋亡相关蛋白（Arc）显着增强突触活动的增加，在成年人的大脑。我们使用免疫细胞化学电子显微镜，以确定是否在发展中的树突的亚细胞定位的弧对应的突触在出生后大鼠尾壳核（CPN）的高峰期。将该分布与μ-阿片样物质受体（M0 R）的分布进行比较，M0 R在树突棘中的定位与出生后发育期间的兴奋性突触形成密切平行（Wang et al. [2003] Neuroscience 118：695-708）。在大鼠CPN突触发生高峰期的开始（出生后第15天; P15）和结束（P30），对Arc或MORs的抗血清进行免疫细胞化学检测。在P15，免疫标记的Arc在树突状细胞的细胞质中呈点状分布，其中一些与多聚核糖体相关。在一些多刺树突，弧免疫反应更强烈地定位在假定的棘比在他们的父母树突，而在其他多刺树突，弧标记限制在轴。许多树突轴和棘也表现出免疫反应性MORs，虽然双重标记的棘比轴少。P30时，单标记和双标记棘的比例分别从2.0%和9.5%增加到7.5%和21%。在突触后致密物下方或质膜上的突触外部位，棘中的弧标记更易检测到。我们的研究结果表明，在出生后发育过程中树突棘的Arc表达和大鼠CPN阿片样物质反应神经元突触发生的发生之间存在相关性。
Dendritic expression of the activity-regulated cytoskeletonassociated protein (Arc) is dramatically enhanced by increased synaptic activity in adult brain. We used immunocytochemical electron microscopy to determine whether the subcellular localization of Arc in developing dendrites corresponds to the peak period of synaptogenesis in the postnatal rat caudate-putamen nucleus (CPN). The distribution was compared with that of mu-opioid receptors (MORs), whose localization in dendritic spines closely parallels excitatory synapse formation during postnatal development (Wang et al.[2003] Neuroscience 118: 695–708). Sections were processed for immunocytochemical detection of antisera against Arc or MORs at the beginning (postnatal day 15; P15) and the end (P30) of the peak period of synaptogenesis in rat CPN. At P15, immunolabeling for Arc showed a punctate distribution in the cytoplasm of dendritic shafts, some of which was associated with polyribosomes. In some spiny dendrites, Arc immunoreactivity was more intensely localized in putative spines than in their parental dendrites, whereas, in other spiny dendrites, Arc labeling was restricted in the shafts. Many dendritic shafts and spines also showed immunoreactivity for MORs, although dually labeled spines were less numerous than the shafts. At P30, the proportion of singly and dually labeled spines significantly increased from 2.0% to 7.5% and from 9.5% to 21%, respectively. Arc labeling in spines was more detectable beneath the postsynaptic density or at extrasynaptic sites on the plasma membrane. Our results suggest a correlation between Arc expression in dendritic spines during postnatal development and the onset of synaptogenesis in opioid-responsive neurons in the rat CPN.