NUMERICAL AND CHEMICAL CLASSIFICATION OF ACTINOPLANES AND SOME RELATED ACTINOMYCETES
NUMERICAL AND CHEMICAL CLASSIFICATION OF ACTINOPLANES AND SOME RELATED ACTINOMYCETES
复制标题
DOI：
10.1099/00221287-136-1-19
复制
发表时间：
1990-01-01
期刊：
影响因子：
--
通讯作者：
MINNIKIN, DE
中科院分区：
文献类型：
--
作者：
GOODFELLOW, M;STANTON, LJ;MINNIKIN, DE
从淡水沉积物中分离出68株游动放线菌，与游动放线菌属、瓶霉属、达氏孢子囊菌属、小单孢菌属、平双孢菌属、螺孢菌属和链孢子囊菌属的标记菌株进行了89个单位性状的比较。采用简单匹配法、Jaccard系数法和模式系数法对数据进行了检验，采用未加权对组法和算术平均法进行了聚类。数值分类仅受所用统计数据或测试误差（估计为5%）的轻微影响。大部分的环境分离物被回收在五个主要的集群，等同于分类种。7个主要的，9个次要的和29个单成员集群定义的SSM系数在83%的相似性（S-）的水平，被分配到两个聚集组外切在68%的S-水平。第一个聚类组由属于游动放线菌属（包括瓶霉属）、Dacquillosporangium和小单孢菌属的菌株组成，第二个聚类组包括Planobispora、Planomonospora、Spirrillospora和Streptosporangium属的代表。经过化学分析的所选游动放线菌菌株含有直链和支链脂肪酸的复杂混合物，具有9个异戊二烯单元的四氢化甲基萘醌作为主要的异戊二烯醇，以及大量的二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油和磷脂酰肌醇;代表性的环境分离物还显示含有内消旋和羟基二氨基庚二酸;主要的壁糖是阿拉伯糖、半乳糖、葡萄糖、甘露糖和木糖。化学和数值分类学数据支持的完整性，除了Actinoplanes caeruleus的既定物种，并显示，五个主要的集群，包括环境隔离物值得排名的物种。建议将这5个新分类群归属于游动放线菌属Actinoplanes consetensis sp. nov.，德文游动放线菌Actinoplanes derwentensis sp. nov.达勒姆游动放线菌Actinoplanes durhamensis sp. nov.潮湿游动放线菌Actinoplanes humidus sp. nov.，和Actinoplanes palleronii sp. nov.。同样，Actinoplanes属、Dacquillussporangium属、Micromonospora属和Pilimelia属也有许多共同的性质，建议将它们归入Micromonosporaceae科Krassilnikov 1938：对该分类群作了订正描述。链孢囊菌科Streptosporangiaceae fam. nov.被提议容纳分配到Microbispora、Microtetraspora、Planobispora、Planomonospora和Streptosporangium属的生物。
Sixty-eight Actinoplanes isolates from freshwater sediments were compared through 89 unit characters with marker strains of the genera Actinoplanes, Ampullariella, Dactylosporangium, Micromonospora, Planobispora, Spirillospora and Streptosporangium. The data were examined using the simple matching, Jaccard and pattern coefficients and clustering was achieved using the unweighted pair group method with arithmetic averages algorithm. The numerical classification was only marginally affected by the statistics used or by test error, estimated at 5%. Most of the environmental isolates were recovered in five major clusters that were equated with taxospecies. Seven major, nine minor and 29 single-member clusters defined by the SSM coefficient at the 83% similarity (S-) level, were assigned to two aggregate groups circumscribed at the 68% S-level. The first cluster group was composed of strains belonging to the genera Actinoplanes (including Ampullariella), Dactylosporangium and Micromonospora and the second included representatives of the genera Planobispora, Planomonospora, Spirillospora and Streptosporangium. Selected Actinoplanes strains subjected to chemical analyses contained complex mixtures of straight- and branched-chain fatty acids, tetrahydrogenated menaquinones with nine isoprene units as the predominant isoprenologue, and major amounts of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol; the representative environmental isolates were also shown to contain meso- and hydroxydiaminopimelic acid; the principal wall sugars were arabinose, galactose, glucose, mannose and xylose. The chemical and numerical taxonomic data support the integrity of established species of Actinoplanes, with the exception of Actinoplanes caeruleus, and show that the five major clusters encompassing environmental isolates merit the rank of species. It is proposed that the five novel taxa be assigned to the genus Actinoplanes as Actinoplanes consettensis sp. nov., Actinoplanes derwentensis sp. nov., Actinoplanes durhamensis sp. nov., Actinoplanes humidus sp. nov., and Actinoplanes palleronii sp. nov. Similarly, the genera Actinoplanes, Dactylosporangium, Micromonospora and Pilimelia have many properties in common and it is proposed that they be classified in the family Micromonosporaceae Krassilnikov 1938: an emended description of this taxon is given. The family Streptosporangiaceae fam. nov. is proposed to accommodate organisms assigned to the genera Microbispora, Microtetraspora, Planobispora, Planomonospora and Streptosporangium.