Analysis of antigen-specific T-Cell responses with synthetic peptides - What kind of peptide for which purpose?
Analysis of antigen-specific T-Cell responses with synthetic peptides - What kind of peptide for which purpose?
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DOI：
10.1016/j.humimm.2004.02.017
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发表时间：
2004-05-01
期刊：
影响因子：
2.7
通讯作者：
Kern, F
中科院分区：
文献类型：
--
作者：
Kiecker, F;Streitz, M;Kern, F
分析t细胞对多肽的反应已成为免疫学研究的一个热门领域。多肽可作为单一刺激物、池或文库使用，或作为多肽/主要组织相容性复合物（MHC）的一部分用于直接t细胞受体染色。为了刺激T细胞，肽必须与MHC分子结合。在这项研究中，我们使用了含有9或10个氨基酸的肽，含有刺激短序列的15个氨基酸的肽，以及带有修饰的c端功能的肽。来自健康巨细胞病毒阳性供体的67176个T细胞结合了常用的巨细胞病毒pp65/HLA-A*0201四聚体，在各自的9个氨基酸肽刺激下能够产生干扰素- γ。在相同浓度下（以马克杯/毫升为单位），长度为15个氨基酸的肽刺激CD8 T细胞的效率略低（平均为各自较短肽诱导频率的77%）。9-氨基酸肽的修饰，如添加氨基酸或官能团，往往导致刺激能力下降。然而，根据我们自己的结果、已发表的数据和理论考虑，我们得出结论，在许多应用中，15个氨基酸长度和11个氨基酸重叠的肽集代表了刺激CD8和CD4 T细胞的良好折衷方案。这些参数可以根据研究的目的进行修改。人类免疫学65,523-536（2004）。(C)美国组织相容性与免疫遗传学学会，2004。Elsevier Inc.出版。
The analysis of T-cell responses to peptides has recently become a busy area of immunologic research. Peptides may be used as single stimulants, pools or libraries, or as part of peptide/major histocompatibility complexes (MHC) for direct T-cell receptor staining. For stimulating T cells, peptides must be bound to MHC molecules. In this study we have used 9- or 10-amino acid peptides, 15-amino acid peptides containing stimulating shorter sequences, and peptides with modified C-terminal function. On average 67176 of the T cells from healthy cytomegalovirus-positive donors that bound a frequently used cytomegalovirus pp65/HLA-A*0201 tetramer were able to produce interferon-gamma on stimulation with the respective 9-amino acid peptide. Peptides of 15 amino acids length used at the same concentration (in mug/ml) stimulated CD8 T cells somewhat less efficiently (on average 77% of the frequencies induced with the respective shorter peptides). Modifications of 9-amino acid peptides such as addition of amino acids or functional groups often resulted in a decreased ability to Stimulate. However, based on our own results, published data, and theoretic considerations, we conclude that sets of peptides of 15 amino acids length with 11 amino acids overlap represent a good compromise for stimulating both CD8 and CD4 T cells in a number of applications. These parameters may be modified subject to the purpose of a study. Human Immunology 65, 523-536 (2004). (C) American Society for Histocompatibility and Immunogenetics, 2004. Published by Elsevier Inc.