Cross-reactive immunity against influenza viruses in children and adults following 2009 pandemic H1N1 infection
Cross-reactive immunity against influenza viruses in children and adults following 2009 pandemic H1N1 infection
复制标题
DOI：
10.1016/j.antiviral.2014.12.008
复制
发表时间：
2015-02-01
影响因子：
7.6
通讯作者：
Zhang, Qibo
中科院分区：
文献类型：
--
作者：
Ahmed, Muhammad S.;Jacques, Laura C.;Zhang, Qibo
2009年H1N1大流行性流感（A（H1N1）pdm 09）病毒感染了全世界大量的人。最近的研究表明，A（H1N1）pdm 09病毒感染引起交叉反应性抗血凝素（HA）记忆B细胞反应的保守区域的HA。然而，A（H1N1）pdm 09感染后儿童和成人交叉反应性免疫的广度和程度尚不清楚。研究方法：我们研究了血清抗HA免疫的一些组-1和-2病毒的儿童和成人使用血凝抑制（HAI），酶联免疫吸附试验和病毒中和试验。结果如下：应用针对A（H1N1）pdm 09的血凝抑制（HAI）滴度>= 40作为血清阳性阈值，我们观察到HAI滴度>= 40的受试者中针对许多病毒亚型（包括中和H5 N1的那些）的抗HA抗体水平显著高于HAI受试者
2009 H1N1 pandemic influenza (A(H1N1)pdm09) virus infected large numbers of people worldwide. Recent studies suggest infection with A(H1N1)pdm09 virus elicited cross-reactive anti-hemagglutinin (HA) memory B cell response to conserved regions of HA. However, the breadth and magnitude of cross-reactive immunity in children and adults following A(H1N1)pdm09 infection are unknown. Methods: We investigated serum anti-HA immunity to a number of group-1 and -2 viruses in children and adults using hemagglutination inhibition (HAI), enzyme-linked immunosorbent assay and virus neutralization assay. Results: Applying hemagglutination inhibition (HAI) titers >= 40 against A(H1N1)pdm09 as threshold of sero-positivity, we observed significantly higher levels of anti-HA antibodies to a number of virus subtypes, including those neutralizing H5N1, in subjects with HAI titer >= 40 than those with HAI