Synthesis and triplex-forming properties of oligonucleotides containing thio-substituted C-nucleoside 4-thiopseudoisocytidine
Synthesis and triplex-forming properties of oligonucleotides containing thio-substituted C-nucleoside 4-thiopseudoisocytidine
复制标题
DOI：
10.1016/j.tetlet.2010.11.065
复制
发表时间：
2011-01-19
影响因子：
1.8
通讯作者：
Sekine, Mitsuo
中科院分区：
文献类型：
--
作者：
Cao, Shi-Qi;Okamoto, Itaru;Sekine, Mitsuo
我们合成了4-硫代异胞苷（s（4）psi iC）的3 '-亚磷酰胺结构单元，并将其掺入形成三链体的寡核苷酸（TF 05）中。掺入s（4）psi iC的三链体的热变性结果表明，s（4）psi iC可用作TFO的核苷组分，以增加三链体在pH 7下的热稳定性。(C)2010爱思唯尔有限公司版权所有。
We synthesized the 3'-phosphoramidite building block of 4-thiopseudoisocytidine (s(4)psi iC) and incorporated it into triplex-forming oligonucleotides (TF05). The results of thermal denaturation of triplexes incorporating s(4)psi iC showed that s(4)psi iC could be used as a nucleoside component of TFOs to increase the thermal stability of triplexes at pH 7. (C) 2010 Elsevier Ltd. All rights reserved.