2b-RAD: a simple and flexible method for genome-wide genotyping
2b-RAD: a simple and flexible method for genome-wide genotyping
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2b-RAD：一种简单而灵活的全基因组基因分型方法
DOI：
10.1038/nmeth.2023
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发表时间：
2012-08-01
期刊：
影响因子：
48
通讯作者：
Matz, Mikhail V.
中科院分区：
文献类型：
--
作者：
Wang, Shi;Meyer, Eli;Matz, Mikhail V.
我们描述了 2b-RAD，这是一种基于对 IIB 型限制性内切酶产生的统一片段进行测序的简化限制性位点相关 DNA (RAD) 基因分型方法。对经过充分研究的拟南芥种质进行基因分型，以验证该方法的准确性并证明根据需要对标记密度进行微调。 2b-RAD 协议的简单性使其特别适合自然群体中连锁图谱和遗传变异分析所需的高通量基因分型。
We describe 2b-RAD, a streamlined restriction site-associated DNA (RAD) genotyping method based on sequencing the uniform fragments produced by type IIB restriction endonucleases. Well-studied accessions of Arabidopsis thaliana were genotyped to validate the method's accuracy and to demonstrate fine-tuning of marker density as needed. The simplicity of the 2b-RAD protocol makes it particularly suitable for high-throughput genotyping as required for linkage mapping and profiling genetic variation in natural populations.