Neurotransmitter phenotype-specific expression changes in developing sympathetic neurons
Neurotransmitter phenotype-specific expression changes in developing sympathetic neurons
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DOI：
10.1016/j.mcn.2007.03.014
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发表时间：
2007-07-01
影响因子：
3.5
通讯作者：
Dechant, Georg
中科院分区：
文献类型：
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作者：
Apostolova, Galina;Dorn, Roland;Dechant, Georg
在晚期发育阶段，交感神经元经历从去甲肾上腺素能神经传递到胆碱能神经传递的转换。这种可塑性现象依赖于体内靶源性信号，并由神经元培养物中的神经营养因子触发。为了分析这两种递质表型之间的全基因组表达差异，我们采用了DNA微阵列。从鸡椎旁交感神经节，神经营养因子3，胶质细胞源性神经营养因子或睫状神经营养因子，所有这些刺激胆碱能分化处理的RNA表达谱。结果与神经生长因子的作用进行了比较，神经生长因子的作用是去甲肾上腺素能刺激。所有三种比较的基因组共同定义了去甲肾上腺素能和胆碱能的共表达组。在这些细胞群中，信号转导、G蛋白偶联信号转导、阳离子转运、神经发生和突触传递等功能类别较为丰富。基于一些已鉴定基因在神经递质开关中起作用的预测的实验将骨形态发生蛋白信号传导鉴定为胆碱能分化的抑制剂。(c)2007爱思唯尔公司All rights reserved.
During late developmental phases individual sympathetic neurons undergo a switch from noradrenergic to cholinergic neurotransmission. This phenomenon of plasticity depends on target-derived signals in vivo and is triggered by neurotrophic factors in neuronal cultures. To analyze genome-wide expression differences between the two transmitter phenotypes we employed DNA microarrays. RNA expression profiles were obtained from chick paravertebral sympathetic ganglia, treated with neurotrophin 3, glial cell line-derived neurotrophic factor or ciliary neurotrophic factor, all of which stimulate cholinergic differentiation. Results were compared with the effect of nerve growth factor, which functions as a pro-noradrenergic stimulus. The gene set common to all three comparisons defined the noradrenergic and cholinergic synexpression groups. Several functional categories, such as signal transduction, G-protein-coupled signaling, cation transport, neurogenesis and synaptic transmission, were enriched in these groups. Experiments based on the prediction that some of the identified genes play a role in the neurotransmitter switch identified bone morphogenetic protein signaling as an inhibitor of cholinergic differentiation. (c) 2007 Elsevier Inc. All rights reserved.