Rapid screening of tissue microarrays for Her-2 fluorescence in situ hybridization testing is an accurate, efficient and economic method of providing an entirely in situ hybridization-based Her-2 testing service
Rapid screening of tissue microarrays for Her-2 fluorescence in situ hybridization testing is an accurate, efficient and economic method of providing an entirely in situ hybridization-based Her-2 testing service
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DOI：
10.1111/j.1365-2559.2009.03257.x
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发表时间：
2009-03-01
期刊：
影响因子：
6.4
通讯作者：
Thomas, Jeremy
中科院分区：
文献类型：
--
作者：
Faratian, Dana;Graham, Ashley;Thomas, Jeremy
荧光原位杂交（FISH）检测是评估乳腺癌患者Her-2状态的“金标准”方法，但由于成本和劳动力方面的考虑，仅在约30%的检测中使用。我们之前描述了基于组织微阵列（TMA）的测试，以消除成本限制，现在描述了一种快速筛选方法，以减少测试时间。我们研究了88例浸润性乳腺癌的TMAs，比较了正式的FISH评分和快速筛查技术。每个核心由两名观察员筛选，结果记录为阳性，模棱两可或阴性。每次接近都是定时的。通过比较快速筛选结果和正式计数来分析数据。使用快速筛查，三分之二的阴性病例和一半的阳性病例可以100%准确地被淘汰。每个观察者在每个案例中花费2分钟快速筛选6个TMA核心，放大倍率为x100。剩下的病例需要正式计数，这并不比使用整段技术花费的时间长。在Her-2 FISH常规检测中快速筛选tma是安全、经济、省时的。该技术确保所有患者接受“金标准”检测。
Fluorescence in situ hybridization (FISH) testing is the 'gold standard' method for Her-2 status assessment in breast cancer patients, yet is only employed in about 30% of tests carried out because of cost and labour considerations. We have previously described tissue microarray (TMA)-based testing to eliminate cost constraints, and now describe a rapid screening approach to reduce time spent testing.We examined 88 cases of invasive breast cancer on TMAs comparing formal FISH scoring with a rapid screening technique. Each core was screened by two observers and results recorded as positive, equivocal or negative. Each approach was timed. Data were analysed by comparing the rapid screening results with formal counts. Using rapid screening, two-thirds of negative and half the positive cases could be eliminated with 100% accuracy. It took 2 min per observer per case to rapid screen six TMA cores at x100 magnification. The remaining cases required formal counting, which took no longer than with whole-section techniques.Rapid screening of TMAs for routine Her-2 FISH testing is safe, economical and time efficient. The technique ensures that all patients receive 'gold standard' testing.