The up-regulation of microRNA-335 is associated with lipid metabolism in liver and white adipose tissue of genetically obese mice
The up-regulation of microRNA-335 is associated with lipid metabolism in liver and white adipose tissue of genetically obese mice
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DOI：
10.1016/j.bbrc.2009.05.058
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发表时间：
2009-08-07
影响因子：
3.1
通讯作者：
Shimano, Hitoshi
中科院分区：
文献类型：
--
作者：
Nakanishi, Noriko;Nakagawa, Yoshimi;Shimano, Hitoshi
MicroRNA（miRNAs）是一类短的非编码RNA，在转录后调节基因表达。一些miRNAs被认为与肥胖有关。然而，与体内肥胖发生相关的miRNAs仍然未知。在这里，我们使用微阵列分析发现肥胖中miR-335的上调。肥胖小鼠（ob/ob、db/db和KKAy小鼠）肝脏和白色脂肪组织（WAT）中miR-335的表达上调。miR-335表达增加与体重、肝脏和WAT重量以及肝脏甘油三酯和胆固醇升高相关。此外，miR-335水平与3 T3-L1脂肪细胞中的脂肪细胞分化标志物如PPAR γ、aP 2和FAS的表达水平密切相关。这些发现首次证明miR-335在肥胖小鼠肝脏和WAT中的表达上调可能参与了肥胖的病理生理过程。(c)2009年由Elsevier Inc.出版
MicroRNAs (miRNAs) are short non-coding RNA that post-transcriptionally regulates gene expression. Some miRNAs have been proposed to be associated with obesity. However, miRNAs, which are related to the development of obesity in vivo remains unknown. Here in, we found the up-regulation of miR-335 in obesity using microarray analysis for miRNA. The expressions of miR-335 in liver and white adipose tissue (WAT) were up-regulated in obese mice including ob/ob, db/db, and KKAy mice. Increased miR-335 expressions were associated with an elevated body, liver and WAT weight, and hepatic triglyceride and cholesterol. Furthermore, miR-335 levels were closely correlated with expression levels of adipocyte differentiation markers such as PPAR gamma, aP2, and FAS in 3T3-L1 adipocyte. These findings provide the first evidence that the up-regulated expressions of miR-335 in liver and WAT of obese mice might contribute to the pathophysiology of obesity. (c) 2009 Published by Elsevier Inc.