Characterization of a Salmonella Enteritidis bacteriophage showing broad lytic activity against Gram-negative enteric bacteria
Characterization of a Salmonella Enteritidis bacteriophage showing broad lytic activity against Gram-negative enteric bacteria
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DOI：
10.1007/s12275-018-8310-1
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发表时间：
2018-12-01
影响因子：
3
通讯作者：
Kim, Jungmin
中科院分区：
文献类型：
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作者：
Kim, Shukho;Kim, Sung-Hun;Kim, Jungmin
在这项研究中，我们试图分离出沙门氏菌特异性裂解噬菌体，我们发现了一个裂解噬菌体，不仅可以裂解沙门氏菌。肠道菌，以及其他革兰氏阴性食源性病原体。这种裂解性噬菌体SS3e可以裂解几乎所有测试的沙门氏菌血清型以及其他肠道致病菌，包括大肠杆菌、宋内志贺氏菌、阴沟肠杆菌和粘质沙雷氏菌。SS 3e噬菌体具有二十面体的头部和长的尾部，表明其属于Siphoviridae。基因组为40，793个碱基对，包含58个理论上确定的开放阅读框（ORF）。在58个ORF中，ORF49和ORF25分别与沙门氏菌噬菌体SE2的尾刺蛋白和溶菌酶样蛋白具有较高的序列相似性，这两个蛋白是识别和裂解宿主菌的关键蛋白。与SE2噬菌体的宿主仅限于肠道沙门氏菌（Salmonella enterica serovars）和鸡沙门氏菌（Gallinarum）不同，SS3e对革兰氏阴性肠道细菌具有更广泛的宿主特异性，因此，它可能是用于对抗包括食源性病原体在内的各种革兰氏阴性细菌感染的噬菌体的有希望的候选者。
In this study, we sought to isolate Salmonella Enteritidis-specific lytic bacteriophages (phages), and we found a lytic phage that could lyse not only S. Enteritidis but also other Gram-negative foodborne pathogens. This lytic phage, SS3e, could lyse almost all tested Salmonella enterica serovars as well as other enteric pathogenic bacteria including Escherichia coli, Shigella sonnei, Enterobacter cloacae, and Serratia marcescens. This SS3e phage has an icosahedral head and a long tail, indicating belong to the Siphoviridae. The genome was 40,793 base pairs, containing 58 theoretically determined open reading frames (ORFs). Among the 58 ORFs, ORF49, and ORF25 showed high sequence similarity with tail spike protein and lysozyme-like protein of Salmonella phage SE2, respectively, which are critical proteins recognizing and lysing host bacteria. Unlike SE2 phage whose host restricted to Salmonella enterica serovars Enteritidis and Gallinarum, SS3e showed broader host specificity against Gram-negative enteric bacteria; thus, it could be a promising candidate for the phage utilization against various Gram-negative bacterial infection including foodborne pathogens.