Making Formalin-Fixed, Paraffin Embedded Blocks
Making Formalin-Fixed, Paraffin Embedded Blocks
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DOI：
10.1007/978-1-4939-8935-5_22
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发表时间：
2019-01-01
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影响因子：
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通讯作者：
Babaheidarian, Pegah
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文献类型：
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作者：
Sadeghipour, Alireza;Babaheidarian, Pegah
石蜡包埋是临床和研究实验室用于创建福尔马林固定的石蜡包埋(FFPE)组织块的标准技术。福尔马林固定的组织经过组织处理，然后包埋在石蜡中，形成FFPE块或石蜡块。石蜡块可以用切片机切割，以生成石蜡中包含的组织的薄片以进行染色，或生成适合于提取核酸的石蜡组织带。此外，FFPE块可以在室温下保存数年。在这里，我们提供了一些基础知识，并介绍了石蜡包埋过程中的常用方法。
Paraffin embedding is a standard technique used in clinical and research laboratories to create a formalin-fixed, paraffin-embedded (FFPE) block of tissue. Formalin-fixed tissue undergoes tissue processing and then is embedded in paraffin (wax) to create a FFPE block or paraffin block. The paraffin block can be cut using a microtome to generate thin sections of tissue contained in paraffin to be stained or paraffin tissue ribbons suitable for nucleic acid extraction. In addition, the FFPE blocks can be stored at room temperature for years. Herein, we provide a basic knowledge, and introduce common methods of the paraffin embedding process.