A Phylogenetic Study of the Gibbons (Hylobates) Using DNA Obtained Noninvasively from Hair
A Phylogenetic Study of the Gibbons (Hylobates) Using DNA Obtained Noninvasively from Hair
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DOI：
10.1016/1055-7903(92)90016-a
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发表时间：
1992-09-01
影响因子：
4.1
通讯作者：
Woodruff, David S.
中科院分区：
文献类型：
--
作者：
Garza, J. Carlos;Woodruff, David S.
描述了23只长臂猿细胞色素b基因252个核苷酸片段的变异。从头发中提取DNA，扩增，并直接测序。这些序列代表了9个命名种中的7个和4个Hylobatid亚属中的3个。在55个地点观察到变异，其中42个具有系统发育信息。分类群之间的过渡性和横向差异水平与其他哺乳动物中报道的同源mtDNA序列相似。简约性、最大似然性和自助性分析(1)支持一些传统的系统发育假说(共色长臂猿的单系)，(2)表明较大物种群体与Hylobates klossi之间以及H.lar与H.Agilis unko之间以前未被认识到的亲缘关系，以及(3)表明该片段不包含在亚属水平上完全解决长臂猿关系的信息。这项研究展示了非侵入性DNA采样在哺乳动物系统发育分析中的巨大潜力。(C)1992年学术出版社，Inc.
Variation in a 252-nucleotide segment of the cytochrome b gene from 23 gibbons is described. DNA was extracted from hair, amplified, and directly sequenced. These sequences represent seven of the nine nominal species and three of the four hylobatid subgenera. Variation was observed at 55 sites, 42 of which are phylogenetically informative. Levels of transitional and transversional divergence between the taxa are similar to those reported for homologous mtDNA sequences in other mammals. Parsimony, maximum likelihood, and bootstrap analyses (1) support some traditional phylogenetic hypotheses (monophyly of the concolor gibbons), (2) suggest previously unrecognized affinities between the lar species group and Hylobates klossi and between H. lar and H. agilis unko, and (3) show that this segment does not contain information sufficient for completely resolving gibbon relationships at the sub-generic level. The study demonstrates the great potential of noninvasive DNA sampling for phylogenetic analyses of mammals. (C) 1992 Academic Press, Inc.