Talin concentrates to the midbody region during mammalian cell cytokinesis
Talin concentrates to the midbody region during mammalian cell cytokinesis
复制标题
DOI：
10.1177/002215549904701102
复制
发表时间：
1999-11-01
影响因子：
3.2
通讯作者：
Simon-Assmann, P
中科院分区：
文献类型：
--
作者：
Bellissent-Waydelich, A;Vanier, MT;Simon-Assmann, P
在这项研究中，我们研究了细胞骨架蛋白talin在哺乳动物细胞胞质分裂过程中的细胞分布。对多种肿瘤细胞系和间充质来源细胞的免疫组化实验表明，talin显示细胞周期依赖性的细胞定位。在中期，talin位于染色体的着丝粒区域，如TD-60蛋白和CREST血清检测到的固有着丝粒成分。从分裂后期到分裂末期，talin都存在于卵裂沟中。随着细胞进行到胞质分裂，当沟完成时，塔林蛋白集中在中间体结构中，如通过免疫荧光评估并通过对纯化的中间体的蛋白质印迹实验证实的。双染色实验表明，α-微管蛋白，TD-60蛋白，和talin共定位在中间体。这些结果表明，talin，除了其在粘着斑组织和信号转导的影响，可能在胞质分裂中发挥关键作用。
In this study we investigated the cellular distribution of talin, a cytoskeletal protein, during mammalian cell cytokinesis. Immunohistochemical experiments on Various carcinoma cell lines and mesenchyme-derived cells reveal that talin displays a cell cycle-dependent cellular localization. During metaphase, talin is located in the centromeric region of the chromosome, like the TD-60 protein and intrinsic centromere components detected by a CREST serum. From anaphase to telophase, talin is present in the cleavage furrow. As the cells progress to cytokinesis, when the furrow is complete, talin is concentrated in the midbody structures, as assessed by immunofluorescence and confirmed by Western blot experiments on purified midbodies. Double staining experiments reveal that a-tubulin, TD-60 protein, and talin co-localize in the midbodies. These results suggest that talin, in addition to its implication in focal adhesion organization and signaling, may play a critical role in cytokinesis.