Phylogenetic study and multiplex PCR-based detection of Burkholderia plantarii, Burkholderia glumae and Burkholderia gladioli using gyrB and rpoD sequences
Phylogenetic study and multiplex PCR-based detection of Burkholderia plantarii, Burkholderia glumae and Burkholderia gladioli using gyrB and rpoD sequences
复制标题
DOI：
10.1099/ijs.0.64184-0
复制
发表时间：
2006-05-01
影响因子：
2.8
通讯作者：
Hikichi, Yasufumi
中科院分区：
文献类型：
--
作者：
Maeda, Yukiko;Shinohara, Hirosuke;Hikichi, Yasufumi
利用gyrB和rpoD的组合核苷酸序列分析了6种植物致病性伯克霍尔德菌的系统发育，建立了植物致病性伯克霍尔德菌（Burkholderia plantariia, glumae Burkholderia gladioli）的检测方法。plantarii、B. glumae和B. gladioli在高自举概率的支持下形成紧密的单系分支。较高的序列相似性揭示了双歧杆菌与植物双歧杆菌的亲缘关系。plantarii菌株被划分为3个由水稻株系组成的substers，而单一的Vanda菌株在系统发育树上占据独特的位置。所有菌株的gyrB和rpoD序列高度保守。相比之下，B. gladioli菌株表现出更大的序列多样性，但这种多样性与菌株的病原、寄主植物或地理来源无关。利用gyrB序列的特异引物，设计了一种多重pcr方法，可以在感染这些致病物种的水稻种子中对B、plantarii、B. glumae和B. gladioli进行特异检测和鉴定。
In order to develop a detection method for the rice pathogens Burkholderia plantarii, Burkholderia glumae and Burkholderia gladioli, the phylogeny of six plant-pathogenic Burkholderia species was analysed using the combined nucleotide sequences of gyrB and rpoD. B. plantarii, B. glumae and B. gladioli formed tight monophyletic branches supported by high bootstrap probabilities. The high sequence similarity revealed a close phylogenetic relationship between B. glumae and B. plantarii. B. plantarii strains were divided into three subdusters comprising rice strains, whereas the single Vanda strain occupied a unique position in the phylogenetic tree. The gyrB and rpoD sequences of all B. glumae strains examined were highly conserved. In contrast, B. gladioli strains demonstrated a far greater sequence diversity, but this diversity did not correlate with pathovar, host plant or geographical origin of the strains. A multiplex-PCR protocol using specific primers from the gyrB sequences was designed that allowed the specific detection and identification of B, plantarii, B. glumae and B. gladioli in rice seeds infected with these pathogenic species.