Nanoscale Arrangement of Proteins by Single-Molecule Cut-and-Paste
Nanoscale Arrangement of Proteins by Single-Molecule Cut-and-Paste
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DOI：
10.1021/ja305689r
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发表时间：
2012-09-19
影响因子：
15
通讯作者：
Gaub, Hermann E.
中科院分区：
文献类型：
--
作者：
Strackharn, Mathias;Pippig, Diana A.;Gaub, Hermann E.
基于蛋白质的纳米结构是生命组织的关键，正是它们的精确排列决定了它们的特定功能。用于蛋白质排列的定向组装的单分子方法允许基于蛋白质组分的系统的受控组成。应用抗体和抗原肽标签，我们利用单分子剪切和粘贴（SMC&P）技术处理单一蛋白质。蛋白质-DNA复合物可以排列成复杂的模式，而蛋白质部分的功能保持不受损害。
Protein-based nanostructures are key to the organization of life and it is their precise arrangement, which determines their specific functions. A single-molecule approach for the directed assembly of protein arrangements allows for a controlled composition of systems based on protein components. Applying antibodies and antigenic peptide tags we utilized the Single-Molecule Cut-and-Paste (SMC&P) technique for the handling of single proteins. Protein-DNA complexes could be arranged to complex patterns with the functionality of the protein part remaining unimpaired.