Sequence of a cDNA coding for mouse manganese superoxide dismutase.
Sequence of a cDNA coding for mouse manganese superoxide dismutase.
复制标题
编码小鼠锰超氧化物歧化酶的 cDNA 序列。
DOI：
10.1093/nar/14.23.9539
复制
发表时间：
1986
影响因子：
14.9
通讯作者：
G. Bell
中科院分区：
文献类型：
--
作者：
R. Hallewell;G. Mullenbach;M. Stempien;G. Bell
锰超氧化物歧化酶(MnSOD)是一种核编码的线粒体酶，可以保护这个细胞器免受超氧阴离子自由基的毒性影响，超氧阴离子自由基是生物氧化的副产物，通过与人的cDNA(2)交叉杂交从Xgt1O的胎盘文库中分离到为鼠酶编码的cDNA(2)；与该探针杂交的125,000个克隆之一。其推导的氨基酸序列与人MnSOD(2，3)有89.6%的同源性：前导肽，514.2%的同源性；成熟蛋白，该克隆缺乏典型的多聚腺苷信号AATAAA，位于Polya区的上游。
Manganese superoxide dismutase (MnSOD) is a nuclear encoded mitochondrial enzyme which protects this organelle from the toxic effects of superoxide radicals that are generated as a by-product of biological oxidations isolated a cDNA coding for the mouse enzyme from a placental library in Xgt1O by cross-hybridization with the human cDNA (2); one of 125,000 clones hyoridized with the probe. The deduced amino acid sequence of the 222 amino acid protein, including a 24 residue leader peptide, possesses 89.6% identity with human MnSOD (2,3):leader peptide, 514.2% identity; mature protein, This clone lacks a canonical polyadenylation signal, AATAAA, upstream of the polyA tract.