The characterization of functions involved in the establishment and maturation of Klebsiella pneumoniae in vitro biofilm reveals dual roles for surface exopolysaccharides
The characterization of functions involved in the establishment and maturation of Klebsiella pneumoniae in vitro biofilm reveals dual roles for surface exopolysaccharides
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DOI：
10.1111/j.1462-2920.2007.01491.x
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发表时间：
2008-03-01
影响因子：
5.1
通讯作者：
Forestier, Christiane
中科院分区：
文献类型：
--
作者：
Balestrino, Damien;Ghigo, Jean-Marc;Forestier, Christiane
形成生物膜的能力被视为致病性和环境性肺炎克雷伯菌菌株中日益重要的定植策略。本研究的目的是在生物膜发育的不同阶段使用不同的模型来鉴定肺炎克雷伯菌的非生物表面定植因子。在静态和动态培养模型中筛选先前通过特征标记诱变获得的 2200 个肺炎克雷伯菌突变体文库，以检测在生物膜形成的早期和/或成熟阶段受损的克隆。共有 28 个突变体在生物膜形成的后期受到影响，而 16 个突变体则表现出早期粘附缺陷。这些突变体对应于潜在细胞和DNA代谢途径以及膜转运功能中涉及的基因。八个突变体缺乏胶囊或 LPS 的产生。基因破坏和显微镜分析表明，LPS 参与玻璃和聚氯乙烯的初始粘附，以及适当初始覆盖基质和构建成熟生物膜结构所需的胶囊。这些结果为与非生物表面定殖能力依次相关的细菌因素提供了新的见解，并揭示了表面胞外多糖在肺炎克雷伯菌生物膜形成过程中发挥的双重作用。
The ability to form biofilm is seen as an increasingly important colonization strategy among both pathogenic and environmental Klebsiella pneumoniae strains. The aim of the present study was to identify abiotic surface colonization factors of K. pneumoniae using different models at different phases of biofilm development. A 2200 K. pneumoniae mutant library previously obtained by signature-tagged mutagenesis was screened in static and dynamic culture models to detect clones impaired at early and/or mature stages of biofilm formation. A total of 28 mutants were affected during late phases of biofilm formation, whereas 16 mutants displayed early adhesion defect. These mutants corresponded to genes involved in potential cellular and DNA metabolism pathways and to membrane transport functions. Eight mutants were deficient in capsule or LPS production. Gene disruption and microscopic analyses showed that LPS is involved in initial adhesion on both glass and polyvinyl-chloride and the capsule required for the appropriate initial coverage of substratum and the construction of mature biofilm architecture. These results give new insight into the bacterial factors sequentially associated with the ability to colonize an abiotic surface and reveal the dual roles played by surface exopolysaccharides during K. pneumoniae biofilm formation.