A Wireless, Regeneratable Cocaine Sensing Scheme Enabled by Allosteric Regulation of pH Sensitive Aptamers.
A Wireless, Regeneratable Cocaine Sensing Scheme Enabled by Allosteric Regulation of pH Sensitive Aptamers.
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DOI：
10.1021/acsnano.2c08511
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发表时间：
2022-12
期刊：
影响因子：
17.1
通讯作者：
Shulin Chen;Tzu-Li Liu;Yan Dong;Jinghua Li
中科院分区：
文献类型：
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作者：
Shulin Chen;Tzu-Li Liu;Yan Dong;Jinghua Li
利用现有技术实现连续生物传感的一个关键挑战是生物识别接口的可重用性差，因为表面饱和时分析物难以从生物受体上解离。在这项工作中，我们介绍了一种可再生的生物传感方案，该方案通过重新设计的 pH 敏感的抗可卡因适体的变构调节来实现。由于质子促进 DNA 构型中的双链体向三链体转变，随后释放吸附的分析物，适体可以恢复与目标分析物的亲和力。放置在传感器旁边的 Pd/PdHx 电极可以通过电化学反应实现局部化学环境的 pH 调节。具有传感和能量收集功能的“花形”、可拉伸、电感耦合电子系统的演示为设计生物集成形式的无线设备提供了一条有前途的途径。这些进步具有未来开发具有片上再生能力的电子传感平台的潜力，可对化学和生物标记物进行连续、定量和实时监测。
A key challenge for achieving continuous biosensing with existing technologies is the poor reusability of the biorecognition interface due to the difficulty in the dissociation of analytes from the bioreceptors upon surface saturation. In this work, we introduce a regeneratable biosensing scheme enabled by allosteric regulation of a re-engineered pH sensitive anti-cocaine aptamer. The aptamer can regain its affinity with target analytes due to proton-promoted duplex-to-triplex transition in DNA configuration followed by the release of adsorbed analytes. A Pd/PdHx electrode placed next to the sensor can enable the pH regulation of the local chemical environment via electrochemical reactions. Demonstration of a "flower-shaped", stretchable, and inductively coupled electronic system with sensing and energy harvesting capabilities provides a promising route to designing wireless devices in biointegrated forms. These advances have the potential for future development of electronic sensing platforms with on-chip regeneration capability for continuous, quantitative, and real-time monitoring of chemical and biological markers.