Protein condensates as aging Maxwell fluids
Protein condensates as aging Maxwell fluids
复制标题
DOI：
10.1126/science.aaw4951
复制
发表时间：
2020-12-11
期刊：
影响因子：
56.9
通讯作者：
Juelicher, Frank
中科院分区：
文献类型：
--
作者：
Jawerth, Louise;Fischer-Friedrich, Elisabeth;Juelicher, Frank
蛋白质凝聚物是一种复杂的流体，可以随着时间的推移改变其材料特性。然而，这些流体的适当的流变学描述仍然缺失。我们使用基于激光镊子的主动和基于微珠的被动流变学表征体外蛋白质凝聚物的时间依赖性材料性质。对于不同的蛋白质，凝聚物的行为在所有年龄的粘弹性麦克斯韦流体。它们的粘度随着年龄的增长而强烈增加，而它们的弹性模量变化很小。在早期和晚期年龄的电子显微镜结构没有显着差异。我们的结论是，蛋白质凝聚物可以是软的玻璃质材料，我们称之为麦克斯韦玻璃与年龄相关的材料特性。我们讨论了玻璃化行为的调制细胞生物化学的可能优势。
Protein condensates are complex fluids that can change their material properties with time. However, an appropriate rheological description of these fluids remains missing. We characterize the time-dependent material properties of in vitro protein condensates using laser tweezer-based active and microbead-based passive rheology. For different proteins, the condensates behave at all ages as viscoelastic Maxwell fluids. Their viscosity strongly increases with age while their elastic modulus varies weakly. No significant differences in structure were seen by electron microscopy at early and late ages. We conclude that protein condensates can be soft glassy materials that we call Maxwell glasses with age-dependent material properties. We discuss possible advantages of glassy behavior for modulation of cellular biochemistry.