Neutrophil transmigration across monolayers of endothelial cells and airway endothelial cells is regulated by different mechanisms
Neutrophil transmigration across monolayers of endothelial cells and airway endothelial cells is regulated by different mechanisms
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DOI：
10.1111/j.1749-6632.1996.tb32563.x
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发表时间：
1996-01-01
期刊：
影响因子：
--
通讯作者：
Knol, EF
中科院分区：
文献类型：
--
作者：
Liu, LX;Mul, FPJ;Knol, EF
中性粒细胞从血流募集到气道是肺部许多炎症性疾病的重要特征之一。在这一过程中，中性粒细胞首先从血液中穿过内皮细胞，沿基底外侧方向进入组织，然后沿基底外侧方向穿过气道上皮，进入肺腔。我们在体外研究并比较了这两种细胞类型在两个方向上跨单层的中性粒细胞迁移的不同机制。当两种细胞类型都生长在滤膜上时，化学引诱剂诱导的中性粒细胞在静息EC上的迁移比在静息上皮细胞上的迁移更强（即从根尖向基底侧方向的迁移）。中性粒细胞在生理的、基底外侧到根尖方向（细胞悬挂在滤过物下面）的迁移比在静止或IL-1 β活化的上皮细胞的相反方向上的迁移要高得多。相比之下，在这些条件下，EC无显著差异。IL-1 β激活4小时后，中性粒细胞在EC中的迁移依赖于IL-8和PAF。然而，跨上皮细胞的迁移在很大程度上依赖于IL-8的产生，而不是PAF。粘附分子ICAM-1对中性粒细胞跨上皮迁移的贡献远小于对内皮细胞迁移的贡献。我们的研究提供了中性粒细胞在体外跨EC和上皮细胞单层迁移的不同机制的证据，表明体内不同的过程控制着中性粒细胞在EC和上皮细胞间的迁移。
Neutrophil recruitment from the blood stream into the airways is one of the important features of many inflammatory disorders in the lung. In this process, neutrophils migrate first from the blood across the ECs in the apical-to-basolateral direction into the tissues and then across airway epithelium in the basolateral-to-apical direction into the lung lumen. We investigated and compared the different mechanisms of neutrophil transmigration in vitro across monolayers of these two cell types in both directions. Neutrophil migration induced by chemoattractants was stronger across resting EC than across resting epithelial cells when both cell types were growing on top of the filters (ie, migration in the apical-to-basolateral direction). Much higher neutrophil transepithelial migration was found in the physiological, basolateral-to-apical direction (cells hanging underneath the filters) than in the opposite direction across either resting or IL-1 beta-activated epithelial cells. In contrast, no significant difference was observed with EC under these conditions. After a 4-h IL-1 beta activation, neutrophil migration across EC was dependent on IL-8 and PAF. However, the migration across epithelial cells relied, to a greater extent, on IL-8 production, and not on PAF. The adhesion molecule ICAM-1 contributed much less to neutrophil migration across epithelium than that across endothelium. Our study provides evidence of different mechanisms of neutrophil transmigration across monolayers of EC and epithelial cells in vitro, indicating that different processes control neutrophil transmigration across EC and epithelial cells in vivo.