Hydrocarbon-utilizing bacteria associated with biofouling materials from offshore waters of the Arabian Gulf
Hydrocarbon-utilizing bacteria associated with biofouling materials from offshore waters of the Arabian Gulf
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DOI：
10.1016/j.ibiod.2011.12.008
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发表时间：
2012-04-01
影响因子：
4.8
通讯作者：
Radwan, S.
中科院分区：
文献类型：
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作者：
Al-Awadhi, H.;Dashti, N.;Radwan, S.
在科威特海岸沿着的8个地点，30个近海基质的生物污损材料含有3 × 10（6）-28 × 10（6）个细菌细胞g（-1），用显微镜计数。使用基于培养的方法，在以油蒸气作为唯一碳源的含氮矿物培养基上计数7 x 10（4）-4.1 x 10（6）菌落形成单位（CFU）。根据16 SrDNA序列分析，这些细菌分属于食烷菌属、迪茨菌属、戈登菌属、Hoyosella属、库克菌属、海蛞蝓属、微球菌属、分枝杆菌属。动球菌属、动微菌属、假交替单胞菌属、盐弧菌属和弧菌属。在无氮矿物培养基上出现了类似数量的CFU：除了土壤杆菌、Labrenzia、微杆菌、Nitratireductor、假单胞菌和红球菌外，优势种隶属于上述记录的大多数属。这些细菌的分离株生长在广泛的个别烷烃，菲，萘，和联苯作为唯一的碳源。定量测定表明，在分批培养中，单个细菌可以使原油、纯正十八烷和纯萘衰减。与新鲜环境样品相关的微生物聚生体也在存在和不存在NaNO 3的情况下在分批培养中衰减油。得出的结论是，与生物污损材料相关的细菌可能有助于阿拉伯湾的碳氢化合物生物修复。(C)2012爱思唯尔有限公司保留所有权利。
Biofouling materials from 30 offshore substrates in eight sites along the Kuwaiti coast harbored 3 x 10(6) -28 x 10(6) bacterial cells g(-1), as counted microscopically. Using culture-based methods, 7 x 10(4)-4.1 x 10(6) colony forming units (CFU's) were counted on a nitrogen-containing mineral medium with oil vapor as a sole carbon source. According to their 16S rDNA sequences, those bacteria were affiliated with species belonging to the genera Alcanivorax, Dietzia, Gordonia, Hoyosella, Kocuria, Marinobacter, Micrococcus, Mycobacterium. Planococcus, Planomicrobium, Pseudoalteromonas, Salinivibrio, and Vibrio. Similar numbers of CFUs appeared on the nitrogen-free mineral media: the predominant species were affiliated with most of the genera recorded above, in addition to Agrobacterium, Labrenzia, Microbacterium, Nitratireductor, Pseudomonas, and Rhodococcus. Isolates of these bacteria grew on a wide range of individual alkanes, phenanthrene, naphthalene, and biphenyl as sole carbon sources. Quantitative determinations showed that individual bacteria could attenuate crude oil, pure n-octadecane, and pure naphthalene in batch cultures. Also microbial consortia associated with fresh environmental samples attenuated oil in batch cultures in the presence and absence of NaNO3. It was concluded that bacteria associated with biofouling materials probably contribute to hydrocarbon bioremediation in the Arabian Gulf. (C) 2012 Elsevier Ltd. All rights reserved.