Functional diversification of CLAVATA3-related CLE proteins in meristem maintenance in rice
Functional diversification of CLAVATA3-related CLE proteins in meristem maintenance in rice
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DOI：
10.1105/tpc.107.057257
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发表时间：
2008-08-01
期刊：
影响因子：
11.6
通讯作者：
Hirano, Hiro-Yuki
中科院分区：
文献类型：
--
作者：
Suzaki, Takuya;Yoshida, Akiko;Hirano, Hiro-Yuki
植物胚后发育取决于茎顶端分生组织（SAM）和根顶端分生组织（RAM）的活性。在拟南芥中，CLAVATA信号通过抑制WUSCHEL负调控SAM中干细胞群体的大小。然而，在其他植物中，对干细胞维持相关因素的研究还不够。在这里，我们报告了两个与CLAVATA 3密切相关的蛋白质，花器官数2（FON2）和FON2样CLE蛋白1（FCP1/OSCLE 402），具有功能多样性，以调节水稻（Oryza sativa）不同类型的分生组织。与FON2不同，FCP1调节花和花序分生组织的维持，FCP1似乎调节营养SAM和RAM的维持。FCP1的组成型表达导致SAM在营养期的消耗，并且FCP1 CLE肽在体外的应用通过RAM中细胞命运的错误指定而干扰根发育。FON1是FON2的一个假定受体，对于这些FCP1功能可能是不必要的。此外，我们确定了一个关键的氨基酸残基，区分FCP1和FON2的行动。我们的研究结果表明，虽然分生组织维持的基本框架是保守的被子植物，在进化过程中的单个因素的功能多样化。
Postembryonic development in plants depends on the activity of the shoot apical meristem (SAM) and root apical meristem (RAM). In Arabidopsis thaliana, CLAVATA signaling negatively regulates the size of the stem cell population in the SAM by repressing WUSCHEL. In other plants, however, studies of factors involved in stem cell maintenance are insufficient. Here, we report that two proteins closely related to CLAVATA3, FLORAL ORGAN NUMBER2 (FON2) and FON2-LIKE CLE PROTEIN1 (FCP1/Os CLE402), have functionally diversified to regulate the different types of meristem in rice (Oryza sativa). Unlike FON2, which regulates the maintenance of flower and inflorescence meristems, FCP1 appears to regulate the maintenance of the vegetative SAM and RAM. Constitutive expression of FCP1 results in consumption of the SAM in the vegetative phase, and application of an FCP1 CLE peptide in vitro disturbs root development by misspecification of cell fates in the RAM. FON1, a putative receptor of FON2, is likely to be unnecessary for these FCP1 functions. Furthermore, we identify a key amino acid residue that discriminates between the actions of FCP1 and FON2. Our results suggest that, although the basic framework of meristem maintenance is conserved in the angiosperms, the functions of the individual factors have diversified during evolution.