Architecture of the Spliceosome
Architecture of the Spliceosome
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DOI：
10.1021/bi201215r
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发表时间：
2012-04-24
期刊：
影响因子：
2.9
通讯作者：
Krummel, Daniel A. Pomeranz
中科院分区：
文献类型：
--
作者：
van der Feltz, Clarisse;Anthony, Kelsey;Krummel, Daniel A. Pomeranz
前体mRNA剪接是由一个非常大的和高度动态的大分子组装称为剪接体催化。详细的剪接体的生化和结构的研究提出了一个艰巨的挑战，但最近已经取得了重大进展，在这方面突出了10亚基的人U1 snRNP的晶体结构。这篇综述概述了我们目前对剪接体的结构和与其功能不可分割的RNA-蛋白质复合物U snRNP的理解。
Precursor-mRNA splicing is catalyzed by an extraordinarily large and highly dynamic macromolecular assemblage termed the spliceosome. Detailed biochemical and structural study of the spliceosome presents a formidable challenge, but there has recently been significant progress made on this front highlighted by the crystal structure of a 10-subunit human U1 snRNP. This review provides an overview of our current understanding of the architecture of the spliceosome and the RNA-protein complexes integral to its function, the U snRNPs.