MGF-19E peptide promoted proliferation, differentiation and mineralization of MC3T3-E1 cell and promoted bone defect healing
MGF-19E peptide promoted proliferation, differentiation and mineralization of MC3T3-E1 cell and promoted bone defect healing
复制标题
MGF-19E肽促进MC3T3-E1细胞增殖、分化和矿化，促进骨缺损愈合
DOI：
10.1016/j.gene.2020.144703
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发表时间：
2020-07-30
期刊：
影响因子：
3.5
通讯作者：
Hao, Linlin
中科院分区：
文献类型：
--
作者：
Wei, Wenzhen;Liu, Songcai;Hao, Linlin
节段性骨缺损和骨折的修复是一项临床挑战，涉及高风险和术后并发症。传统植骨方式的骨损伤和部分骨肿瘤切除术后并发症较多。生长因子已被提出作为替代方案来促进骨修复和形成，并绕过这些限制。在本研究中，我们对不同物种中不同长度的机械生长因子(MGF)E肽进行了分类，并分析了它们对MC3T3-E1细胞增殖、细胞周期、碱性磷酸酶(ALP)活性、分化相关因子表达和细胞矿化的影响。建立兔骨损伤模型，通过注射候选MGF E多肽来验证其修复功能。我们分析了52种不同的MGF-E多肽，并将其分为以下四类：T-MGF-25e、M-MGF-25e、T-MGF-19e和M-MGF-19e。这些多肽的合成为进一步研究奠定了基础。培养72 h后，T-MGF-19E通过调节细胞周期明显促进细胞增殖，其中T-MGF-25E和T-MGF-19E在第14天促进成骨细胞分化，M-MGF-25E在第7天促进细胞分化，T-MGF-19E、T-MGF-25E和M-MGF-19E对成骨细胞矿化有明显促进作用，其中T-MGF19E作用最显著。提示T-MGF19E多肽能显著促进MC3T3-E1细胞的增殖、分化和矿化。在兔骨缺损模型上，小剂量T-MGF-19E多肽对骨损伤愈合有明显的促进作用，提示其对骨损伤愈合有促进作用。
The repair of segmental bone defects and bone fractures is a clinical challenge involving high risk and postsurgical morbidity. Bone injury and partial bone tumor resection via traditional bone grafting result in high complications. Growth factors have been proposed as alternatives to promote bone repair and formation and circumvent these limitations. In this study, we classified different lengths of mechano growth factor (MGF) E peptides in different species and analyzed their effects on MC3T3-E1 cell proliferation, cell cycle, alkaline phosphatase (ALP) activity, differentiation-related factor expression, and cell mineralization. A rabbit bone injury model was constructed, and the repair function of MGF E peptide was verified by injecting the candidate MGF E peptide. We analyzed 52 different MGF-E peptides and classified them into the following four categories: T-MGF-25E, M-MGF-25E, T-MGF-19E, and M-MGF-19E. These peptides were synthesized for further study. T-MGF-19E peptide obviously promoted cell proliferation by regulating cell cycle after MGF E peptide treatment at 72 h. T-MGF-25E and T-MGF-19E peptide significantly promoted the differentiation of osteoblasts on day 14, and M-MGF-25E peptide promoted cell differentiation on day 7. T-MGF-19E, T-MGF-25E, and M-MGF-19E significantly promoted osteoblast mineralization, with T-MGF19E showing the most significant effect. These results implied that T-MGF19E peptide could remarkably promote MC3T3-E1 cell proliferation, differentiation, and mineralization. The rabbit bone defect model showed that the low-dose T-MGF-19E peptide significantly promoted bone injury healing, suggesting its promoting effect on the healing of bone injury.