Evaluation of high-risk human papillomaviruses type distribution in cervical cancer in Sichuan province of China.
Evaluation of high-risk human papillomaviruses type distribution in cervical cancer in Sichuan province of China.
复制标题
四川省宫颈癌高危型人乳头瘤病毒型别分布评价
DOI：
10.1186/1471-2407-8-202
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发表时间：
2008-07-22
期刊：
影响因子：
3.8
通讯作者：
Zha X
中科院分区：
文献类型：
--
作者：
Wu EQ;Zhang GN;Yu XH;Ren Y;Fan Y;Wu YG;Kong W;Zha X
背景：高危人乳头瘤病毒感染是宫颈癌发生的重要因素，HPV类型在世界范围内的分布差异很大。确定特定类型的人乳头瘤病毒流行率是研制预防宫颈癌疫苗的重要一步。方法采用MY09/11一致引物PCR （MY09/11 PCR）、型特异引物一步PCR （one-step TS PCR）和E6/E7基因型特异引物巢式PCR （nested TS PCR）相结合的方法检测190例中国西南人口大省四川省宫颈癌标本中的人乳头瘤病毒（HPV）基因型。我们调查了HPV在宫颈癌患者中的患病率和分布，特别是HPV 16、18、52、58和59型，怀疑在中国某些地区最常见。结果my09 - 11 PCR、一步式TS PCR和巢式TS PCR检出HPV感染率分别为159例（83.7%）、145例（76.3%）和172例（90.5%）。HPV总患病率为93.2%（177/190）。单步TS-PCR检测HPV16、18、52、58和59阳性标本分别为111例（58.4%）、14例（7.4%）、6例（3.2%）、13例（6.8%）和4例（2.1%）。巢式TS-PCR检测HPV16、52、58和59的检出率分别为140（73.7%）、30（15.8%）、37（19.5%）和25(13.2%)，而HPV18的检出率仅为4（2.1%）。结论我们的数据显示，在中国四川地区宫颈癌妇女中，除HPV 16型最常见外，HPV 58型、52型和59型比HPV18型更常见。
BackgroundInfection with high-risk human papillomavirus is an important factor associated with cervical cancer, and the distribution of HPV types varies greatly worldwide. Determination of type-specific HPV prevalence constitutes an important step towards the development of vaccines for the prevention of cervical cancer.MethodsThe human papillomavirus (HPV) genotypes in 190 cervical cancer specimens taken from the Sichuan province, the most populous province of Southwest China, were detected by a combination of MY09/11 consensus primers PCR (MY09/11 PCR), type-specific primers one-step PCR (One-step TS PCR) and E6/E7 gene type-specific primers nested PCR (Nested TS PCR). The prevalence and distribution of HPV in patients with cervical cancer, especially for HPV types 16, 18, 52, 58 and 59, suspected to be most common in certain parts of China, was investigated.ResultsThe HPV infection rates detected by MY09/11 PCR, One-step TS PCR and Nested TS PCR were 159 (83.7%), 145 (76.3%) and 172 (90.5%), respectively. The overall HPV prevalence was 93.2% (177/190). The positive specimens for HPV16, 18, 52, 58 and 59 detected by One-step TS-PCR were 111 (58.4%), 14 (7.4%), 6 (3.2%), 13 (6.8%) and 4 (2.1%), respectively. By Nested TS-PCR analysis, the detection rates of HPV16, 52, 58 and 59 were increased to 140 (73.7%), 30 (15.8%), 37 (19.5%) and 25 (13.2%), while only 4 (2.1%) additional specimens were found to be infected with HPV18.ConclusionOur data demonstrate that, besides HPV 16, which was found to be the most prevalent type, HPV types 58, 52 and 59 are more prevalent than HPV18 in women with cervical cancer in the Sichuan area of China.