Role of extra- and intracellular Ca2+ in the lymphatic myogenic response
Role of extra- and intracellular Ca2+ in the lymphatic myogenic response
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DOI：
10.1152/ajpregu.1997.272.1.r326
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发表时间：
1997-01-01
影响因子：
2.8
通讯作者：
Johnston, MG
中科院分区：
文献类型：
--
作者：
Atchison, DJ;Johnston, MG
我们使用一种主动收缩的牛肠系膜淋巴管的体外制备方法来研究选择的钙通道调节剂对这些血管推进液体的能力的影响。我们发现，用硝苯地平和地尔硫卓阻断二氢吡啶受体，在临床使用的浓度范围内(分别为10(-7)和10(-6)M)，可抑制泵送。使用兰诺定(10(-6)M)的细胞内钙调节也抑制了泵。此外，我们还研究了这些药物对淋巴流量和跨壁压力之间的关系的影响，这种关系通常用钟形曲线来描述。地尔硫卓，10(-6)M，在所研究的压力范围内衰减泵送。另一方面，10(-7)M的兰诺定浓度能够抑制恒定跨壁压力下的泵送，当操纵跨壁压力时，对压力-流量关系没有影响。因此，我们已经确定，通过L通道的钙运动对淋巴泵活性的调节有重要作用，而细胞内钙通量的作用较小，可能被跨壁压力所改变。
We used an actively contracting in vitro preparation of bovine mesenteric lymph vessels to study the effect of selected calcium channel modulators on the ability of these vessels to propel fluid. We found that blocking the dihydropyridine receptor with nifedipine and diltiazem inhibited pumping at concentrations within the range used clinically (10(-7) and 10(-6) M, respectively). Intracellular calcium modulation using ryanodine (10(-6) M) also inhibited pumping. Furthermore, we studied the effect of these agents on the relationship between lymph flow and transmural pressure, a relationship normally described by a bell-shaped curve. Diltiazem, 10(-6) M, attenuated pumping over the range of pressures studied. On the other hand, ryanodine at 10(-7) M, a concentration capable of inhibiting pumping at a constant transmural pressure, had no effect on the pressure-flow relationship when transmural pressure was manipulated. Thus we have determined that calcium movement via the L-type channel contributes significantly to the regulation of lymph pump activity and that intracellular calcium flux plays a less significant role that may be modified by transmural pressure.