MicroRNA expression differentiates between primary lung tumors and metastases to the lung
MicroRNA expression differentiates between primary lung tumors and metastases to the lung
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DOI：
10.1016/j.prp.2010.03.005
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Perelman, Marina
中科院分区：
文献类型：
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作者：
Barshack, Iris;Lithwick-Yanai, Gila;Perelman, Marina
对于外科病理学家来说，区分肺肿瘤是原发性还是转移性是具有挑战性的，而且目前的生物标志物并不总是有助于肺肿瘤的分类。microRNA的组织相关表达可能解释了许多肿瘤可以仅根据其microRNA表达特征进行分类的显著发现。本研究表明，microRNAs可以作为肺肿瘤分类的生物标志物。利用76例福尔马林固定石蜡包埋（FFPE）原发性肺癌或肺转移性肿瘤的microRNA微阵列数据，我们确定了一组microRNA在这两组之间表达差异。这一组包括在肺原发肿瘤中过表达最强烈的hsa-miR-182和在转移性肿瘤中过表达的hsa-miR-126。用qRT-PCR在54个样本上证实了这组microrna的差异表达。根据我们的数据，microRNA的表达应该被视为外科病理学家在识别难以理解的肺肿瘤类型时的潜在临床生物标志物。(C) 2010 Elsevier GmbH版权所有。
For surgical pathologists, distinguishing whether a pulmonary neoplasm is primary or metastatic can be challenging, and current biomarkers do not always aid lung tumor classification. The tissue-associated expression of microRNA likely explains the remarkable finding that many tumors can be classified based solely on their microRNA expression signature. Here we show that microRNAs can serve as biomarkers for lung tumor classification. Using microRNA microarray data generated from 76 formalin-fixed, paraffin-embedded (FFPE) samples of either primary lung cancer or metastatic tumors to the lung, we have identified a set of microRNAs expressed differentially between these two groups. This set includes hsa-miR-182, which was most strongly over-expressed in the lung primary tumors, and hsa-miR-126, which was over-expressed in the metastatic tumors. The differential expression of this set of microRNAs was confirmed using qRT-PCR on a set of 54 samples. In light of our data, microRNA expression should be considered as a potential clinical biomarker for surgical pathologists faced with discerning the tumor type of an inscrutable lung neoplasm. (C) 2010 Elsevier GmbH. All rights reserved.