Imaging analysis and selected sequence monitoring of small peptides using planar chromatography/secondary ion mass spectrometry.
Imaging analysis and selected sequence monitoring of small peptides using planar chromatography/secondary ion mass spectrometry.
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使用平面色谱/二次离子质谱法对小肽进行成像分析和选定序列监测。
DOI：
10.1002/bms.1200170510
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Busch,KL
中科院分区：
文献类型：
--
作者：
Dunphy,JC;Busch,KL
通过二次离子质谱对硅胶薄层色谱上所含的脑啡肽和缓激肽的混合物进行成像。使用精密操纵器确定色谱平面中样品分布的空间分布，该操纵器将样品移入和移出液态金属镓源中产生的主光束。例如，通过监测每种化合物的质子化分子 [M + H]+，或者在选定的序列监测实验中，通过监测每个肽组成员共有的序列离子，可以方便地测量空间分布。后一个实验可用于快速定位和鉴定通过平面色谱（包括薄层色谱和电泳）分离的一类肽。
Mixtures of enkephalins and bradykinins contained on silica gel thin‐layer chromatograms were imaged by secondary ion mass spectrometry. Spatial profiles of the sample distribution in the chromatographic plane were determined using a precision manipulator that moves the sample into and out of the primary beam generated in a liquid metal gallium source. For instance, spatial profiles are conveniently measured by monitoring the protonated molecule [M + H]+of each compound or, in a selected‐sequence monitoring experiment, by monitoring a sequence ion common to the members of each peptide group. The latter experiment is useful for quick location and identification of a class of peptides separated by planar chromatogrpahy, including thin‐layer chromatography and electrophoresis.