Expression of collagenase-1 (MMP-1), collagenase-3 (MMP-13) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in laryngeal squamous cell carcinomas
Expression of collagenase-1 (MMP-1), collagenase-3 (MMP-13) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in laryngeal squamous cell carcinomas
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DOI：
10.1007/s00405-003-0610-2
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发表时间：
2003-10-01
影响因子：
2.6
通讯作者：
Zatonski, T
中科院分区：
文献类型：
--
作者：
Krecicki, T;Fraczek, M;Zatonski, T
基质金属蛋白酶（MMPs）具有抗细胞外基质（ECM）成分的蛋白水解活性，在肿瘤的侵袭性和转移性扩散中起重要作用。MMPs和MMPs的组织抑制剂（TIMPs）在喉鳞状细胞癌（LSCC）中的作用尚未得到充分阐明。本研究的目的是评估胶原酶-1 （MMP-1）、胶原酶-3 （MMP-13）和TIMP-1的表达与LSCCs临床病理特征的相关性。用免疫组织化学方法检测50例手术获得的原发性LSCCs标本中胶原酶和TIMP-1的表达。分析表明，LSCC细胞和基质细胞均表达MMP-1、MMP-13和TIMP-1免疫染色。TIMP-1过表达在非转移性病例中更为常见（P =0.009）。TIMP-1和MMP-1染色与LSCC的组织学类型有显著相关性。角化型癌的TIMP-1蛋白表达高于非角化型癌（P =0.01）。TIMP-1染色与分化程度相关，因为它主要存在于分化良好和中度的癌中（P =0.04）。研究结果证实，所分析的MMPs和TIMP-1的表达是LSCC的特征，并且这些酶有助于肿瘤的进展。TIMP-1上调可能在LSCCs中表现出较低的转移潜力，并且与肿瘤进展的早期阶段有关。TIMP-1的表达似乎也依赖于分化的程度。
Matrix metalloproteinases (MMPs) that have a proteolytic activity against the components of extracellular matrix (ECM) play an important role in the invasive and metastatic spread of tumors. The role of MMPs and tissue inhibitors of MMPs (TIMPs) in laryngeal squamous cell carcinoma (LSCC) has not been elucidated sufficiently. The aim of the present study was to evaluate the correlation between the expression of collagenase-1 (MMP-1), collagenase-3 (MMP-13) and TIMP-1, as well as the clinicopathological features of LSCCs. The expression of collagenases and TIMP-1 was examined immunohistochemically in 50 cases of surgically obtained specimens of primary LSCCs. Analyses indicated that LSCC cells as well as stromal cells expressed MMP-1, MMP-13 and TIMP-1 immunostaining. Overexpression of TIMP-1 occurred more frequently in non-metastasizing cases ( P =0.009). TIMP-1 and MMP-1 staining correlated significantly with the histologic type of LSCC. The keratinizing type of carcinomas exhibited higher TIMP-1 protein expression than the nonkeratinizing variety ( P =0.01). TIMP-1 staining was associated with the grade of differentiation, since it was found predominantly in well and moderately differentiated carcinomas ( P =0.04). The findings confirm that expression of analyzed MMPs and TIMP-1 is characteristic of LSCC and that these enzymes contribute to the progression of tumors. TIMP-1 upregulation might exhibit lower metastatic potential in LSCCs and is linked rather with an early stage of tumor progression. It seems also that TIMP-1 expression is dependent on the grade of differentiation.