Combinatorial microarray analysis revealing Arabidopsis genes implicated in cytokinin responses through the His→Asp phosphorelay circuitry
Combinatorial microarray analysis revealing Arabidopsis genes implicated in cytokinin responses through the His→Asp phosphorelay circuitry
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DOI：
10.1093/pcp/pci033
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发表时间：
2005-02-01
影响因子：
4.9
通讯作者：
Mizuno, T
中科院分区：
文献类型：
--
作者：
Kiba, T;Naitou, T;Mizuno, T
在拟南芥中，植物对细胞分裂素的即时早期反应被表述为多步组氨酸激酶（AHP）-->含组氨酸磷酸传递素（AHP）-->反应调节子（ARR）磷酸传递信号通路，该通路由细胞分裂素受体组氨酸蛋白激酶启动。为了找到在细胞分裂素介导的His -> Asp磷粘土信号通路下游起作用的组分（或基因），我们进行了全基因组微阵列分析。为此，我们使用了一种组合微阵列策略，不仅采用野生型植物，而且采用了某些转基因株系，其中细胞分裂素介导的His -> Asp磷酸化信号通路已被遗传操纵。所用的这些转基因株系是ARR 21过表达和ARR 22过表达的植物，其每一个都表现出关于细胞分裂素介导的His -> Asp磷酸化传递的特征表型。对这些植物进行广泛的微阵列分析的结果使我们能够系统地鉴定出一定数量的基因，这些基因在转录水平上直接或间接地响应于细胞分裂素而上调。其中，在野生型植物中进一步检查了一些代表，以支持以下观点：某些编码转录因子的基因在转录水平上被细胞分裂素以类似于A型ARR基因的方式快速且特异性地诱导，这是His -> Asp磷酸化信号通路的标志。因此，几个感兴趣的转录因子被鉴定为细胞分裂素应答性的，包括属于AP 2/EREBP家族、MYB家族、加塔家族或bHLH家族的那些。包括这些在内，所列出的细胞分裂素上调基因（214个）将为我们理解A. thaliana.
In Arabidopsis thaliana, the immediate early response of plants to cytokinin is formulated as the multistep histidine kinase (AHP) --> histidine-containing phosphotransmitter (AHP) --> response regulator (ARR) phosphorelay signaling circuitry, which is initiated by the cytokinin receptor histidine protein kinases. In the hope of finding components (or genes) that function downstream of the cytokinin-mediated His --> Asp phosphorclay signaling circuitry, we carried out genome-wide microarray analyses. To this end, we used a combinatorial microarray strategy by employing not only wild-type plants, but also certain transgenic lines in which the cytokinin-mediated His --> Asp phosphorelay signaling circuitry has been genetically manipulated. These transgenic lines employed were ARR21-overexpressing and ARR22-overexpressing plants, each of which exhibits a characteristic phenotype with regard to the cytokinin-mediated His --> Asp phosphorelay. The results of extensive microarray analyses with these plants allowed us systematically to identify a certain number of genes that were up-regulated at the level of transcription in response to cytokinin directly or indirectly. Among them, some representatives were examined further in wild-type plants to support the idea that certain genes encoding transcription factors are rapidly and specifically induced at the level of transcription by cytokinin in a manner similar to that of the type-A ARR genes, which are the hallmarks of the His --> Asp phosphorelay signaling circuitry. Several interesting transcription factors were thus identified as being cytokinin responsive, including those belonging to the AP2/EREBP family, MYB family, GATA family or bHLH family. Including these, the presented list of cytokinin-up-regulated genes (214) will provide us with valuable bases for understanding the His --> Asp phosphorelay in A. thaliana.